제품 > 항체 > 다중클론항체(pAb)

ZNF645 Rabbit pAb (A18553)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - ZNF645 Rabbit pAb (A18553)

Western blot analysis of various lysates using ZNF645 Rabbit pAb (A18553) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product nameZNF645 Rabbit pAb
Catalog No.A18553
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the zinc finger domain-containing protein family. This family member contains both a RING-type and a C2H2-type of zinc finger domain, and it may function as an E3 ubiquitin-protein ligase. Protein localization suggests a role in human sperm production and quality control.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 346-425 of human ZNF645 (NP_689790.1).
SequenceQNGNPSASEFASHHYNLNILPQFTENQETLSPQFTQTDAMDHRRWPAWKRLSPCPPTRSPPPSTLHGRSHHSHQRRHRRY
Gene ID
Swiss Prot
SynonymsCT138; HAKAIL; ZNF645
Calculated MW49kDa
Observed MW49kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse testis, Rat testis
Cellular locationcytoplasm

Documents

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Lot number

    ABclonal:Western blot - ZNF645 Rabbit pAb (A18553)}

    Western blot - ZNF645 Rabbit pAb (A18553)

    Western blot analysis of various lysates using ZNF645 Rabbit pAb (A18553) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.

    * For research use only. Not for therapeutic or diagnostic purposes.