제품 > 항체 > 다중클론항체(pAb)

ZNF703 Rabbit pAb (A14325)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ZNF703 Rabbit pAb (A14325)

Western blot analysis of lysates from MCF7 cells, using ZNF703 Rabbit pAb (A14325) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - ZNF703 Rabbit pAb (A14325)

Immunohistochemistry analysis of paraffin-embedded rat pancreas using ZNF703 Rabbit pAb (A14325) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ZNF703 Rabbit pAb (A14325)

Immunohistochemistry analysis of paraffin-embedded human breast cancer using ZNF703 Rabbit pAb (A14325) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameZNF703 Rabbit pAb
Catalog No.A14325
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable DNA-binding transcription factor binding activity. Involved in several processes, including cellular response to estradiol stimulus; mammary gland epithelial cell differentiation; and positive regulation of mammary gland epithelial cell proliferation. Located in cytoplasm and nuclear matrix. Part of protein-containing complex.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 160-290 of human ZNF703 (NP_079345.1).
SequenceGSGGGDSRKDSGSSSVSSTSSSSSSSPGDKAGFRVPSAACPPFPPHGAPVSASSSSSSPGGSRGGSPHHSDCKNGGGVGGGELDKKDQEPKPSPEPAAVSRGGGGEPGAHGGAESGASGRKSEPPSALVGA
Gene ID
Swiss Prot
SynonymsNLZ1; ZPO1; ZEPPO1; ZNF503L; ZNF703
Calculated MW58kDa
Observed MW58kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMCF7
Cellular locationCytoplasm, Nucleus

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - ZNF703 Rabbit pAb (A14325)}

    Western blot - ZNF703 Rabbit pAb (A14325)

    Western blot analysis of lysates from MCF7 cells, using ZNF703 Rabbit pAb (A14325) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunohistochemistry - ZNF703 Rabbit pAb (A14325)}

    Immunohistochemistry - ZNF703 Rabbit pAb (A14325)

    Immunohistochemistry analysis of paraffin-embedded rat pancreas using ZNF703 Rabbit pAb (A14325) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - ZNF703 Rabbit pAb (A14325)}

    Immunohistochemistry - ZNF703 Rabbit pAb (A14325)

    Immunohistochemistry analysis of paraffin-embedded human breast cancer using ZNF703 Rabbit pAb (A14325) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)