제품 > 항체 > 다중클론항체(pAb)

ZO-1 Rabbit pAb (A11417)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using ZO-1 Rabbit pAb (A11417) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - ZO-1 Rabbit pAb (A11417)

Immunofluorescence analysis of Mouse colon tissue using ZO-1 Rabbit pAb (A11417) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.

Review

Overview

Product name	ZO-1 Rabbit pAb
Catalog No.	A11417
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family of proteins, and acts as a tight junction adaptor protein that also regulates adherens junctions. Tight junctions regulate the movement of ions and macromolecules between endothelial and epithelial cells. The multidomain structure of this scaffold protein, including a postsynaptic density 95/disc-large/zona occludens (PDZ) domain, a Src homology (SH3) domain, a guanylate kinase (GuK) domain and unique (U) motifs all help to co-ordinate binding of transmembrane proteins, cytosolic proteins, and F-actin, which are required for tight junction function. Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 18-273 of human ZO-1 (NP_003248.3).
Sequence	IWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPD
Gene ID
Swiss Prot
Synonyms	ZO-1
Calculated MW	195kDa
Observed MW	250kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	293T, Mouse heart
Cellular location	Cell junction, Cell membrane, Cytoplasmic side, Peripheral membrane protein, gap junction, tight junction
Customer validation	(Pig cells) WB(Sus scrofa, Mus musculus, Homo sapiens) IF(Gallus gallus) Other(Macaca mulatta)

Documents

Certificate of Compliance

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Lot number

Research Area

ZO-1 Rabbit pAb images

Western blot - ZO-1 Rabbit pAb (A11417)

Immunofluorescence - ZO-1 Rabbit pAb (A11417)

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A11417

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Reviews List

Product Protocols

References for ZO-1 Rabbit pAb (A11417)

Product:

ZO-1 Rabbit pAb

Journal:

Frontiers in microbiology

Application:

IF:

6.1

Species:

Sus scrofa

PMID:

29867808

Title:

Lactobacillus frumenti Facilitates Intestinal Epithelial Barrier Function Maintenance in Early-Weaned Piglets

References for ZO-1 Rabbit pAb (A11417)

Product:

ZO-1 Rabbit pAb

Journal:

Frontiers in Microbiology

Application:

IF:

6.1

Species:

Mus musculus

PMID:

32296403

Title:

Obacunone Protects Against Ulcerative Colitis in Mice by Modulating Gut Microbiota, Attenuating TLR4/NF-κB Signaling Cascades, and Improving Disrupted Epithelial Barriers

References for ZO-1 Rabbit pAb (A11417)

Product:

ZO-1 Rabbit pAb

Journal:

Frontiers in nutrition

Application:

IF:

Species:

Mus musculus

PMID:

36313077

Title:

Collagen peptide promotes DSS-induced colitis by disturbing gut microbiota and regulation of macrophage polarization - PMC

References for ZO-1 Rabbit pAb (A11417)

Product:

ZO-1 Rabbit pAb

Journal:

Journal of hazardous materials

Application:

IF:

12.2

Species:

Gallus gallus

PMID:

36423455

Title:

Thiram-induced hyperglycemia causes tibial dyschondroplasia by triggering aberrant ECM remodeling via the gut-pancreas axis in broiler chickens

References for ZO-1 Rabbit pAb (A11417)

Product:

ZO-1 Rabbit pAb

Journal:

Scientific reports

Application:

IF:

3.8

Species:

Mus musculus

PMID:

37095192

Title:

Gut microbiota induces hepatic steatosis by modulating the T cells balance in high fructose diet mice - PMC

References for ZO-1 Rabbit pAb (A11417)

Product:

ZO-1 Rabbit pAb

Journal:

Microbiome

Application:

IF:

13.8

Species:

Mus musculus

PMID:

37158970

Title:

Gut microbiota-derived 3-phenylpropionic acid promotes intestinal epithelial barrier function via AhR signaling - PMC

References for ZO-1 Rabbit pAb (A11417)

Product:

ZO-1 Rabbit pAb

Journal:

Current research in food science

Application:

IF:

6.3

Species:

Mus musculus

PMID:

37351541

Title:

Lactoferrin alleviates Western diet-induced cognitive impairment through the microbiome-gut-brain axis - PMC

References for ZO-1 Rabbit pAb (A11417)

Product:

ZO-1 Rabbit pAb

Journal:

Toxicology

Application:

IF:

4.8

Species:

Homo sapiens

PMID:

37442268

Title:

Selenium nanoparticles alleviate deoxynivalenol-induced intestinal epithelial barrier dysfunction by regulating endoplasmic reticulum stress in IPEC-J2 cells

References for ZO-1 Rabbit pAb (A11417)

Product:

ZO-1 Rabbit pAb

Journal:

The ISME journal

Application:

IF:

10.8

Species:

Mus musculus

PMID:

38366194

Title:

Characterizing core microbiota and regulatory functions of the pig gut microbiome - PMC

References for ZO-1 Rabbit pAb (A11417)

Product:

ZO-1 Rabbit pAb

Journal:

Frontiers in microbiology

Application:

IF:

6.1

Species:

Mus musculus

PMID:

35572623

Title:

The Metabolites of Lactobacillus fermentum F-B9-1 Relieved Dextran Sulfate Sodium-Induced Experimental Ulcerative Colitis in Mice

References for ZO-1 Rabbit pAb (A11417)

Product:

ZO-1 Rabbit pAb

Journal:

Front Pharmacol

Application:

IF:

5.6

Species:

Mus musculus

PMID:

34795594

Title:

Berberine Improves Irinotecan-Induced Intestinal Mucositis Without Impairing the Anti-colorectal Cancer Efficacy of Irinotecan by Inhibiting Bacterial -glucuronidase

References for ZO-1 Rabbit pAb (A11417)

Product:

ZO-1 Rabbit pAb

Journal:

International journal of biological macromolecules

Application:

IF:

7.7

Species:

Mus musculus

PMID:

38710247

Title:

Fucoidan from Apostichopus japonicus ameliorates alcoholic liver disease by regulating gut-liver axis homeostasis

References for ZO-1 Rabbit pAb (A11417)

Product:

ZO-1 Rabbit pAb

Journal:

Toxics

Application:

IF:

4.6

Species:

Mus musculus

PMID:

38787118

Title:

Integrating Epigenetics, Proteomics, and Metabolomics to Reveal the Involvement of Wnt/-Catenin Signaling Pathway in Oridonin-Induced Reproductive Toxicity - PMC

References for ZO-1 Rabbit pAb (A11417)

Product:

ZO-1 Rabbit pAb

Journal:

Journal of agricultural and food chemistry

Application:

Other

IF:

5.7

Species:

Macaca mulatta

PMID:

38871671

Title:

Ameliorated Effects of Fucoidan on Dextran Sulfate Sodium-Induced Ulcerative Colitis and Accompanying Anxiety and Depressive Behaviors in Aged C57BL/6 Mice

References for ZO-1 Rabbit pAb (A11417)

Product:

ZO-1 Rabbit pAb

Journal:

Pesticide biochemistry and physiology

Application:

IF:

4.2

Species:

Mus musculus

PMID:

39084803

Title:

Thiram exposure: Disruption of the blood-testis barrier and altered apoptosis-autophagy dynamics in testicular cells via the Bcl-2/Bax and mTOR/Atg5/p62 pathways in

References for ZO-1 Rabbit pAb (A11417)

Product:

ZO-1 Rabbit pAb

Journal:

Cell biology and toxicology

Application:

IF:

5.3

Species:

Mus musculus

PMID:

39422738

Title:

Three bioactive compounds from Huangqin decoction ameliorate Irinotecan-induced diarrhea via dual-targeting of Escherichia coli and bacterial -glucuronidase - PMC

References for ZO-1 Rabbit pAb (A11417)

Product:

ZO-1 Rabbit pAb

Journal:

Biomolecules

Application:

IF:

4.8

Species:

Homo sapiens

PMID:

39595632