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ZRANB3 Rabbit pAb (A9555)

Publications (4) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ZRANB3 Rabbit pAb (A9555)

Western blot analysis of various lysates using ZRANB3 Rabbit pAb (A9555) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

Overview

Product nameZRANB3 Rabbit pAb
Catalog No.A9555
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables ATP-dependent DNA/DNA annealing activity; K63-linked polyubiquitin modification-dependent protein binding activity; and endodeoxyribonuclease activity. Involved in several processes, including DNA metabolic process; DNA rewinding; and negative regulation of DNA recombination. Located in nuclear replication fork and nucleoplasm.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 818-1077 of human ZRANB3 (NP_001273497.1).
SequenceITKQQTKQNCTKRYITKEDVAVASMDKVKNVGGHVRLITKESRPRDPFTKKLLEDGACVPFLNPYTVQADLTVKPSTSKGYLQAVDNEGNPLCLRCQQPTCQTKQACKANSWDSRFCSLKCQEEFWIRSNNSYLRAKVFETEHGVCQLCNVNAQELFLRLRDAPKSQRKNLLYATWTSKLPLEQLNEMIRNPGEGHFWQVDHIKPVYGGGGQCSLDNLQTLCTVCHKERTARQAKERSQVRRQSLASKHGSDITRFLVKK
Gene ID
Swiss Prot
SynonymsAH2; 4933425L19Rik; ZRANB3
Calculated MW123kDa
Observed MW123kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:3000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, Raji, Jurkat, 293T, Mouse liver, Mouse heart, Mouse brain, Rat liver
Cellular locationChromosome, Nucleus
Customer validation

WB(Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

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    ABclonal:Western blot - ZRANB3 Rabbit pAb (A9555)}

    Western blot - ZRANB3 Rabbit pAb (A9555)

    Western blot analysis of various lysates using ZRANB3 Rabbit pAb (A9555) at 1:3000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 20s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A9555? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)