제품 > 항체 > 다중클론항체(pAb)

Zfp541 Rabbit pAb (A15530)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - Zfp541 Rabbit pAb (A15530)

Western blot analysis of various lysates using Zfp541 Rabbit pAb (A15530) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product nameZfp541 Rabbit pAb
Catalog No.A15530
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable transcription corepressor activity. Predicted to be involved in histone deacetylation; negative regulation of transcription, DNA-templated; and regulation of transcription by RNA polymerase II. Predicted to act upstream of or within cell differentiation and spermatogenesis. Located in nucleus. Part of histone deacetylase complex. Orthologous to human ZNF541 (zinc finger protein 541).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-300 of mouse Zfp541 (NP_001092747.1).
SequenceMEPYSLGEEGALPSEGHLPSFSESQVLNCSDTLNRDLGPSTRDLLYAGLSGLDLDPSLSTSDMPSEVLEDNLDTLSLYSGKDSDSVKLLEEYADSESQTSLQDLGLGALKVPKEADEGGRATGSTRKGKRQHSSPQNPLLDCSLCGKVFSSASSLSKHYLTHSQERKHVCKVCSKAFKRQDHLTGHMLTHQKTKPFVCIEQGCSKSYCDYRSLRRHYEVQHGVCILKETPPEEEAYGDPTHNHDVANQPPPSGLRSLGPPEARSPGSVLPNRDLLRCIVSSIVHQKIPSPGPAVGPSDTE
Gene ID
Swiss Prot
SynonymsZnf541; EG666528; Zfp541
Calculated MW141kDa/148kDa
Observed MW170kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:200 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesmouse brain, mouse testis, rat brain, rat testis
Cellular locationNucleus

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    ABclonal:Western blot - Zfp541 Rabbit pAb (A15530)}

    Western blot - Zfp541 Rabbit pAb (A15530)

    Western blot analysis of various lysates using Zfp541 Rabbit pAb (A15530) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)