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Zinc-α2-glycoprotein (ZAG/AZGP1) Rabbit mAb (A11680)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - Zinc-α2-glycoprotein (ZAG/AZGP1) Rabbit mAb (A11680)

Western blot analysis of lysates from Rat plasma, using Zinc-α2-glycoprotein (ZAG/AZGP1) Rabbit mAb (A11680) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Immunohistochemistry - Zinc-α2-glycoprotein (ZAG/AZGP1) Rabbit mAb (A11680)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using Zinc-α2-glycoprotein (ZAG/AZGP1) Rabbit mAb (A11680) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameZinc-α2-glycoprotein (ZAG/AZGP1) Rabbit mAb
Catalog No.A11680
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0673
Involved in cell adhesion and detection of chemical stimulus involved in sensory perception of bitter taste. Located in extracellular space.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human Zinc-α2-glycoprotein (ZAG/AZGP1) (P25311).
SequenceMVRMVPVLLSLLLLLGPAVPQENQDGRYSLTYIYTGLSKHVEDVPAFQALGSLNDLQFFRYNSKDRKSQPMGLWRQVEGMEDWKQDSQLQKAREDIFMET
Gene ID
Swiss Prot
SynonymsZAG; ZA2G; Zinc-α2-glycoprotein (ZAG/AZGP1)
Calculated MW34kDa
Observed MW45kDa
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesRat plasma
Cellular locationSecreted

Documents

Certificate of Compliance

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    ABclonal:Western blot - Zinc-α2-glycoprotein (ZAG/AZGP1) Rabbit mAb (A11680)}

    Western blot - Zinc-α2-glycoprotein (ZAG/AZGP1) Rabbit mAb (A11680)

    Western blot analysis of lysates from Rat plasma, using Zinc-α2-glycoprotein (ZAG/AZGP1) Rabbit mAb (A11680) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 3min.
    ABclonal:Immunohistochemistry - Zinc-α2-glycoprotein (ZAG/AZGP1) Rabbit mAb (A11680)}

    Immunohistochemistry - Zinc-α2-glycoprotein (ZAG/AZGP1) Rabbit mAb (A11680)

    Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using Zinc-α2-glycoprotein (ZAG/AZGP1) Rabbit mAb (A11680) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.

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