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Zinc-α2-glycoprotein (ZAG/AZGP1) Rabbit pAb (A5365)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - Zinc-α2-glycoprotein (ZAG/AZGP1) Rabbit pAb (A5365)

Western blot analysis of extracts of human serum, using Zinc-α2-glycoprotein (ZAG/Zinc-α2-glycoprotein (ZAG/AZGP1)) antibody (A5365) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product nameZinc-α2-glycoprotein (ZAG/AZGP1) Rabbit pAb
Catalog No.A5365
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Involved in cell adhesion and detection of chemical stimulus involved in sensory perception of bitter taste. Located in extracellular space.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 21-298 of human Zinc-α2-glycoprotein (ZAG/Zinc-α2-glycoprotein (ZAG/AZGP1)) (NP_001176.1).
SequenceQENQDGRYSLTYIYTGLSKHVEDVPAFQALGSLNDLQFFRYNSKDRKSQPMGLWRQVEGMEDWKQDSQLQKAREDIFMETLKDIVEYYNDSNGSHVLQGRFGCEIENNRSSGAFWKYYYDGKDYIEFNKEIPAWVPFDPAAQITKQKWEAEPVYVQRAKAYLEEECPATLRKYLKYSKNILDRQDPPSVVVTSHQAPGEKKKLKCLAYDFYPGKIDVHWTRAGEVQEPELRGDVLHNGNGTYQSWVVVAVPPQDTAPYSCHVQHSSLAQPLVVPWEAS
Gene ID
Swiss Prot
SynonymsZAG; ZA2G
Calculated MW34kDa
Observed MW41kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHuman serum
Cellular locationSecreted
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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    ABclonal:Western blot - Zinc-α2-glycoprotein (ZAG/AZGP1) Rabbit pAb (A5365)}

    Western blot - Zinc-α2-glycoprotein (ZAG/AZGP1) Rabbit pAb (A5365)

    Western blot analysis of extracts of human serum, using Zinc-α2-glycoprotein (ZAG/Zinc-α2-glycoprotein (ZAG/AZGP1)) antibody (A5365) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A5365? Please let us know so that we can cite the reference in this datasheet.

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    Secondary Antibodies (26)