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β-arrestin1 Rabbit pAb (A0998)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - β-arrestin1 Rabbit pAb (A0998)

Western blot analysis of various lysates using β-arrestin1 Rabbit pAb (A0998) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - β-arrestin1 Rabbit pAb (A0998)

Immunohistochemistry analysis of paraffin-embedded Rat heart using β-arrestin1 Rabbit pAb (A0998) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - β-arrestin1 Rabbit pAb (A0998)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using β-arrestin1 Rabbit pAb (A0998) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - β-arrestin1 Rabbit pAb (A0998)

Immunofluorescence analysis of A-549 cells using β-arrestin1 Rabbit pAb (A0998) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - β-arrestin1 Rabbit pAb (A0998)

Immunofluorescence analysis of HepG2 cells using β-arrestin1 Rabbit pAb (A0998) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - β-arrestin1 Rabbit pAb (A0998)

Immunofluorescence analysis of NIH/3T3 cells using β-arrestin1 Rabbit pAb (A0998) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - β-arrestin1 Rabbit pAb (A0998)

Immunofluorescence analysis of PC-12 cells using β-arrestin1 Rabbit pAb (A0998) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - β-arrestin1 Rabbit pAb (A0998)

Immunoprecipitation analysis of 200 μg extracts of SH-SY5Y cells using 3 μg β-arrestin1 antibody (A0998). Western blot was performed from the immunoprecipitate using β-arrestin1 (A0998) at a dilution of 1:1000.

Overview

Product nameβ-arrestin1 Rabbit pAb
Catalog No.A0998
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 1 is a cytosolic protein and acts as a cofactor in the beta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Besides the central nervous system, it is expressed at high levels in peripheral blood leukocytes, and thus the BARK/beta-arrestin system is believed to play a major role in regulating receptor-mediated immune functions. Alternatively spliced transcripts encoding different isoforms of arrestin beta 1 have been described.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 169-418 of human β-arrestin1 (NP_004032.2).
SequenceRKVQYAPERPGPQPTAETTRQFLMSDKPLHLEASLDKEIYYHGEPISVNVHVTNNTNKTVKKIKISVRQYADICLFNTAQYKCPVAMEEADDTVAPSSTFCKVYTLTPFLANNREKRGLALDGKLKHEDTNLASSTLLREGANREILGIIVSYKVKVKLVVSRGGLLGDLASSDVAVELPFTLMHPKPKEEPPHREVPENETPVDTNLIELDTNDDDIVFEDFARQRLKGMKDDKEEEEDGTGSPQLNNR
Gene ID
Swiss Prot
SynonymsARB1; ARR1; β-arrestin1
Calculated MW47kDa
Observed MW51kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesK-562, A-549, Mouse lung, Rat lung, Rat brain
Cellular locationCell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Membrane, Nucleus, clathrin-coated pit, pseudopodium.
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - β-arrestin1 Rabbit pAb (A0998)}

Western blot - β-arrestin1 Rabbit pAb (A0998)

Western blot analysis of various lysates using β-arrestin1 Rabbit pAb (A0998) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - β-arrestin1 Rabbit pAb (A0998)}

Immunohistochemistry - β-arrestin1 Rabbit pAb (A0998)

Immunohistochemistry analysis of paraffin-embedded Rat heart using β-arrestin1 Rabbit pAb (A0998) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - β-arrestin1 Rabbit pAb (A0998)}

Immunohistochemistry - β-arrestin1 Rabbit pAb (A0998)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using β-arrestin1 Rabbit pAb (A0998) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - β-arrestin1 Rabbit pAb (A0998)}

Immunofluorescence - β-arrestin1 Rabbit pAb (A0998)

Immunofluorescence analysis of A-549 cells using β-arrestin1 Rabbit pAb (A0998) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - β-arrestin1 Rabbit pAb (A0998)}

Immunofluorescence - β-arrestin1 Rabbit pAb (A0998)

Immunofluorescence analysis of HepG2 cells using β-arrestin1 Rabbit pAb (A0998) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - β-arrestin1 Rabbit pAb (A0998)}

Immunofluorescence - β-arrestin1 Rabbit pAb (A0998)

Immunofluorescence analysis of NIH/3T3 cells using β-arrestin1 Rabbit pAb (A0998) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - β-arrestin1 Rabbit pAb (A0998)}

Immunofluorescence - β-arrestin1 Rabbit pAb (A0998)

Immunofluorescence analysis of PC-12 cells using β-arrestin1 Rabbit pAb (A0998) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - β-arrestin1 Rabbit pAb (A0998)}

Immunoprecipitation - β-arrestin1 Rabbit pAb (A0998)

Immunoprecipitation analysis of 200 μg extracts of SH-SY5Y cells using 3 μg β-arrestin1 antibody (A0998). Western blot was performed from the immunoprecipitate using β-arrestin1 (A0998) at a dilution of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A0998? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

Secondary Antibodies (26)