제품 > 항체 > 단일클론항체(mAb)

beta glucuronidase (GUSB) Rabbit mAb (A19248)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - beta glucuronidase (GUSB) Rabbit mAb (A19248)

Western blot analysis of extracts of various cell lines, using beta glucuronidase (beta glucuronidase (GUSB)) antibody (A19248) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product namebeta glucuronidase (GUSB) Rabbit mAb
Catalog No.A19248
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2408
This gene encodes a hydrolase that degrades glycosaminoglycans, including heparan sulfate, dermatan sulfate, and chondroitin-4, 6-sulfate. The enzyme forms a homotetramer that is localized to the lysosome. Mutations in this gene result in mucopolysaccharidosis type VII. Alternative splicing results in multiple transcript variants. There are many pseudogenes of this locus in the human genome.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human beta glucuronidase (GUSB) (NP_000172.2).
SequenceMARGSAVAWAALGPLLWGCALGLQGGMLYPQESPSRECKELDGLWSFRADFSDNRRRGFEEQWYRRPLWESGPTVDMPVPSSFNDISQDWRLRHFVGWVW
Gene ID
Swiss Prot
SynonymsBG; MPS7
Calculated MW75kDa
Observed MW75kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHepG2, A-549, HL-60
Cellular locationLysosome

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - beta glucuronidase (GUSB) Rabbit mAb (A19248)}

    Western blot - beta glucuronidase (GUSB) Rabbit mAb (A19248)

    Western blot analysis of extracts of various cell lines, using beta glucuronidase (beta glucuronidase (GUSB)) antibody (A19248) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)