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eEF1A1 Rabbit mAb (A23515)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - eEF1A1 Rabbit mAb (A23515)

Western blot analysis of various lysates, using eEF1A1 Rabbit mAb (A23515) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.2s.

ABclonal:Immunohistochemistry - eEF1A1 Rabbit mAb (A23515)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using eEF1A1 Rabbit mAb (A23515) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - eEF1A1 Rabbit mAb (A23515)

Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma tissue using eEF1A1 Rabbit mAb (A23515) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - eEF1A1 Rabbit mAb (A23515)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using eEF1A1 Rabbit mAb (A23515) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - eEF1A1 Rabbit mAb (A23515)

Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using eEF1A1 Rabbit mAb (A23515) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - eEF1A1 Rabbit mAb (A23515)

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using eEF1A1 Rabbit mAb (A23515) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - eEF1A1 Rabbit mAb (A23515)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using eEF1A1 Rabbit mAb (A23515) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - eEF1A1 Rabbit mAb (A23515)

Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using eEF1A1 Rabbit mAb (A23515) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - eEF1A1 Rabbit mAb (A23515)

Immunofluorescence analysis of C6 cells using eEF1A1 Rabbit mAb (A23515) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameeEF1A1 Rabbit mAb
Catalog No.A23515
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC55875
This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 300-400 of human eEF1A1. (NP_001393.1).
SequenceSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDGPKFLKSGDAA
Gene ID
Swiss Prot
SynonymsCCS3; EF1A; PTI1; CCS-3; EE1A1; EEF-1; EEF1A; EF-Tu; EF1A1; LENG7; eEF1A-1; GRAF-1EF; EF1alpha1; eEF1A1
Calculated MW50kDa
Observed MW50kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesK-562, HeLa, Mouse thymus, PC-12
Cellular locationCytoplasm, Nucleus, nucleolus
Customer validation

WB(Mus musculus, Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - eEF1A1 Rabbit mAb (A23515)}

Western blot - eEF1A1 Rabbit mAb (A23515)

Western blot analysis of various lysates, using eEF1A1 Rabbit mAb (A23515) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.2s.
ABclonal:Immunohistochemistry - eEF1A1 Rabbit mAb (A23515)}

Immunohistochemistry - eEF1A1 Rabbit mAb (A23515)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using eEF1A1 Rabbit mAb (A23515) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - eEF1A1 Rabbit mAb (A23515)}

Immunohistochemistry - eEF1A1 Rabbit mAb (A23515)

Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma tissue using eEF1A1 Rabbit mAb (A23515) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - eEF1A1 Rabbit mAb (A23515)}

Immunohistochemistry - eEF1A1 Rabbit mAb (A23515)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using eEF1A1 Rabbit mAb (A23515) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - eEF1A1 Rabbit mAb (A23515)}

Immunohistochemistry - eEF1A1 Rabbit mAb (A23515)

Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using eEF1A1 Rabbit mAb (A23515) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - eEF1A1 Rabbit mAb (A23515)}

Immunohistochemistry - eEF1A1 Rabbit mAb (A23515)

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using eEF1A1 Rabbit mAb (A23515) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - eEF1A1 Rabbit mAb (A23515)}

Immunohistochemistry - eEF1A1 Rabbit mAb (A23515)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using eEF1A1 Rabbit mAb (A23515) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - eEF1A1 Rabbit mAb (A23515)}

Immunohistochemistry - eEF1A1 Rabbit mAb (A23515)

Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using eEF1A1 Rabbit mAb (A23515) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - eEF1A1 Rabbit mAb (A23515)}

Immunofluorescence - eEF1A1 Rabbit mAb (A23515)

Immunofluorescence analysis of C6 cells using eEF1A1 Rabbit mAb (A23515) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A23515? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

Secondary Antibodies (26)