Western blot - eEF1A1 Rabbit pAb (A17857)
Western blot analysis of various lysates using eEF1A1 Rabbit pAb (A17857) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Western blot - eEF1A1 Rabbit pAb (A17857)
Western blot analysis of various lysates using eEF1A1 Rabbit pAb (A17857) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunohistochemistry - eEF1A1 Rabbit pAb (A17857)
Immunohistochemistry analysis of paraffin-embedded mouse brain using eEF1A1 Rabbit pAb (A17857) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - eEF1A1 Rabbit pAb (A17857)
Immunohistochemistry analysis of paraffin-embedded mouse kidney using eEF1A1 Rabbit pAb (A17857) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - eEF1A1 Rabbit pAb (A17857)
Immunohistochemistry analysis of paraffin-embedded rat ovary using eEF1A1 Rabbit pAb (A17857) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence - eEF1A1 Rabbit pAb (A17857)
Immunofluorescence analysis of Raw264.7 cells using eEF1A1 Rabbit pAb (A17857) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence - eEF1A1 Rabbit pAb (A17857)
Immunofluorescence analysis of A-549 cells using eEF1A1 Rabbit pAb (A17857) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence - eEF1A1 Rabbit pAb (A17857)
Immunofluorescence analysis of C6 cells using eEF1A1 Rabbit pAb (A17857) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence - eEF1A1 Rabbit pAb (A17857)
Immunofluorescence analysis of HeLa cells using eEF1A1 Rabbit pAb (A17857) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence - eEF1A1 Rabbit pAb (A17857)
Immunofluorescence analysis of NIH/3T3 cells using eEF1A1 Rabbit pAb (A17857) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence - eEF1A1 Rabbit pAb (A17857)
Immunofluorescence analysis of U2OS cells using eEF1A1 Rabbit pAb (A17857) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.