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eIF2A Rabbit mAb (A9709)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - eIF2A Rabbit mAb (A9709)

Western blot analysis of various lysates using eIF2A Rabbit mAb (A9709) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - eIF2A Rabbit mAb (A9709)

Western blot analysis of lysates from Rat lung, using eIF2A Rabbit mAb (A9709) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

ABclonal:Immunohistochemistry - eIF2A Rabbit mAb (A9709)

Immunohistochemistry analysis of paraffin-embedded Rat pancreas using eIF2A Rabbit mAb (A9709) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - eIF2A Rabbit mAb (A9709)

Immunohistochemistry analysis of paraffin-embedded Mouse testis using eIF2A Rabbit mAb (A9709) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - eIF2A Rabbit mAb (A9709)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using eIF2A Rabbit mAb (A9709) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - eIF2A Rabbit mAb (A9709)

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using eIF2A Rabbit mAb (A9709) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - eIF2A Rabbit mAb (A9709)

Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using eIF2A Rabbit mAb (A9709) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Overview

Product nameeIF2A Rabbit mAb
Catalog No.A9709
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1712
This gene encodes a eukaryotic translation initiation factor that catalyzes the formation of puromycin-sensitive 80 S preinitiation complexes and the poly(U)-directed synthesis of polyphenylalanine at low concentrations of Mg2+. This gene should not be confused with eIF2-alpha (EIF2S1, Gene ID: 1965), the alpha subunit of the eIF2 translation initiation complex. Although both of these proteins function in binding initiator tRNA to the 40 S ribosomal subunit, the encoded protein does so in a codon-dependent manner, whereas eIF2 complex requires GTP. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 450-550 of human eIF2A (Q9BY44).
SequenceALRNKPITNSKLHEEEPPQNMKPQSGNDKPLSKTALKNQRKHEAKKAAKQEARSDKSPDLAPTPAPQSTPRNTVSQSISGDPEIDKKIKNLKKKLKAIEQL
Gene ID
Swiss Prot
SynonymsCDA02; EIF-2A; MST089; MSTP004; MSTP089; eIF2A
Calculated MW65kDa
Observed MW60kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:200 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesBxPC-3, Mouse liver, Rat lung
Cellular locationblood microparticle, cytoplasm, eukaryotic translation initiation factor 2 complex, extracellular space.
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - eIF2A Rabbit mAb (A9709)}

    Western blot - eIF2A Rabbit mAb (A9709)

    Western blot analysis of various lysates using eIF2A Rabbit mAb (A9709) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Western blot - eIF2A Rabbit mAb (A9709)}

    Western blot - eIF2A Rabbit mAb (A9709)

    Western blot analysis of lysates from Rat lung, using eIF2A Rabbit mAb (A9709) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 3min.
    ABclonal:Immunohistochemistry - eIF2A Rabbit mAb (A9709)}

    Immunohistochemistry - eIF2A Rabbit mAb (A9709)

    Immunohistochemistry analysis of paraffin-embedded Rat pancreas using eIF2A Rabbit mAb (A9709) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - eIF2A Rabbit mAb (A9709)}

    Immunohistochemistry - eIF2A Rabbit mAb (A9709)

    Immunohistochemistry analysis of paraffin-embedded Mouse testis using eIF2A Rabbit mAb (A9709) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - eIF2A Rabbit mAb (A9709)}

    Immunohistochemistry - eIF2A Rabbit mAb (A9709)

    Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using eIF2A Rabbit mAb (A9709) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - eIF2A Rabbit mAb (A9709)}

    Immunohistochemistry - eIF2A Rabbit mAb (A9709)

    Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using eIF2A Rabbit mAb (A9709) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - eIF2A Rabbit mAb (A9709)}

    Immunohistochemistry - eIF2A Rabbit mAb (A9709)

    Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using eIF2A Rabbit mAb (A9709) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A9709? Please let us know so that we can cite the reference in this datasheet.

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    Secondary Antibodies (26)