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eIF4A3 Rabbit mAb (A8985)

Publications (4) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - eIF4A3 Rabbit mAb (A8985)

Western blot analysis of extracts of various cell lines, using eIF4A3 Rabbit mAb (A8985) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Immunofluorescence - eIF4A3 Rabbit mAb (A8985)

Immunofluorescence analysis of U-2 OS cells using eIF4A3 Rabbit mAb (A8985) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameeIF4A3 Rabbit mAb
Catalog No.A8985
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1373
This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a nuclear matrix protein. Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4AI and eIF4AII, two other members of the DEAD box protein family.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 34-125 of human eIF4A3 (P38919).
SequenceVDVTPTFDTMGLREDLLRGIYAYGFEKPSAIQQRAIKQIIKGRDVIAQSQSGTGKTATFSISVLQCLDIQVRETQALILAPTRELAVQIQKG
Gene ID
Swiss Prot
SynonymsFal1; RCPS; DDX48; MUK34; NUK34; NMP265; eIF4AIII; eIF4A-III; eIF-4A-III
Calculated MW47kDa
Observed MW50kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesPC-12, MCF7, Mouse thymus, Rat testis
Cellular locationCytoplasm, Nucleus, Nucleus speckle
Customer validation

IF(Homo sapiens)

IP(Homo sapiens)

WB(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - eIF4A3 Rabbit mAb (A8985)}

Western blot - eIF4A3 Rabbit mAb (A8985)

Western blot analysis of extracts of various cell lines, using eIF4A3 Rabbit mAb (A8985) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
ABclonal:Immunofluorescence - eIF4A3 Rabbit mAb (A8985)}

Immunofluorescence - eIF4A3 Rabbit mAb (A8985)

Immunofluorescence analysis of U-2 OS cells using eIF4A3 Rabbit mAb (A8985) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A8985? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)