제품 > 항체 > 단일클론항체(mAb)

gamma-Synuclein Rabbit mAb (A2735)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using gamma-Synuclein Rabbit mAb (A2735) at 1：1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Immunohistochemistry - gamma-Synuclein Rabbit mAb (A2735)

Immunohistochemistry analysis of paraffin-embedded rat brain using gamma-Synuclein Rabbit mAb (A2735) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product name	gamma-Synuclein Rabbit mAb
Catalog No.	A2735
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC0762

Background

This gene encodes a member of the synuclein family of proteins which are believed to be involved in the pathogenesis of neurodegenerative diseases. Mutations in this gene have also been associated with breast tumor development.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 48-127 of human gamma-Synuclein (O76070).
Sequence	VVQSVTSVAEKTKEQANAVSEAVVSSVNTVATKTVEEAENIAVTSGVVRKEDLRPSAPQQEGEASKEKEEVAEEAQSGGD
Gene ID
Swiss Prot
Synonyms	SR; BCSG1; gamma-Synuclein
Calculated MW	13kDa
Observed MW	16kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	HT-29, BxPC-3, Mouse spleen
Cellular location	Cytoplasm, centrosome, cytoskeleton, microtubule organizing center, perinuclear region, spindle