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hnRNP A1 Rabbit mAb (A11564)

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - hnRNP A1 Rabbit mAb (A11564)

Western blot analysis of various lysates using hnRNP A1 Rabbit mAb (A11564) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - hnRNP A1 Rabbit mAb (A11564)

Western blot analysis of various lysates using hnRNP A1 Rabbit mAb (A11564) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Immunohistochemistry - hnRNP A1 Rabbit mAb (A11564)

Immunohistochemistry analysis of paraffin-embedded Human colon using hnRNP A1 Rabbit mAb (A11564) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - hnRNP A1 Rabbit mAb (A11564)

Confocal imaging of U-2 OS cells using hnRNP A1 Rabbit mAb (A11564, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - hnRNP A1 Rabbit mAb (A11564)

Confocal imaging of NIH/3T3 cells using hnRNP A1 Rabbit mAb (A11564, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - hnRNP A1 Rabbit mAb (A11564)

Confocal imaging of C6 cells using hnRNP A1 Rabbit mAb (A11564, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunoprecipitation - hnRNP A1 Rabbit mAb (A11564)

Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg hnRNP A1 antibody (A11564). Western blot was performed from the immunoprecipitate using hnRNP A1 antibody (A11564) at a dilution of 1:1000.

Overview

Product namehnRNP A1 Rabbit mAb
Catalog No.A11564
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0633
This gene encodes a member of a family of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs), which are RNA-binding proteins that associate with pre-mRNAs in the nucleus and influence pre-mRNA processing, as well as other aspects of mRNA metabolism and transport. The protein encoded by this gene is one of the most abundant core proteins of hnRNP complexes and plays a key role in the regulation of alternative splicing. Mutations in this gene have been observed in individuals with amyotrophic lateral sclerosis 20. Multiple alternatively spliced transcript variants have been found. There are numerous pseudogenes of this gene distributed throughout the genome.hnRNP A1 has three isoforms with MW 30 kDa, 34 kDa and 39 kDa.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 250-350 of human hnRNP A1 (P09651).
SequenceDGGYGGGGPGYSGGSRGYGSGGQGYGNQGSGYGGSGSYDSYNNGGGGGFGGGSGSNFGGGGSYNDFGNYNNQSSNFGPMKGGNFGGRSSGPYGGGGQYFAK
Gene ID
Swiss Prot
SynonymsUP 1; ALS19; ALS20; HNRPA1; IBMPFD3; HNRPA1L3; hnRNP A1; hnRNP-A1
Calculated MW39kDa
Observed MW30kDa/34kDa/39kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IHC-P 1:200 - 1:2000
  • IF/ICC 1:200 - 1:2000
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, 293T, HepG2, Mouse brain, Rat brain
Cellular locationCytoplasm, Nucleus.
Customer validation

WB(Homo sapiens)

IP(Homo sapiens)

IF(Homo sapiens)

Documents

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ABclonal:Western blot - hnRNP A1 Rabbit mAb (A11564)}

Western blot - hnRNP A1 Rabbit mAb (A11564)

Western blot analysis of various lysates using hnRNP A1 Rabbit mAb (A11564) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - hnRNP A1 Rabbit mAb (A11564)}

Western blot - hnRNP A1 Rabbit mAb (A11564)

Western blot analysis of various lysates using hnRNP A1 Rabbit mAb (A11564) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
ABclonal:Immunohistochemistry - hnRNP A1 Rabbit mAb (A11564)}

Immunohistochemistry - hnRNP A1 Rabbit mAb (A11564)

Immunohistochemistry analysis of paraffin-embedded Human colon using hnRNP A1 Rabbit mAb (A11564) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - hnRNP A1 Rabbit mAb (A11564)}

Immunofluorescence - hnRNP A1 Rabbit mAb (A11564)

Confocal imaging of U-2 OS cells using hnRNP A1 Rabbit mAb (A11564, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - hnRNP A1 Rabbit mAb (A11564)}

Immunofluorescence - hnRNP A1 Rabbit mAb (A11564)

Confocal imaging of NIH/3T3 cells using hnRNP A1 Rabbit mAb (A11564, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - hnRNP A1 Rabbit mAb (A11564)}

Immunofluorescence - hnRNP A1 Rabbit mAb (A11564)

Confocal imaging of C6 cells using hnRNP A1 Rabbit mAb (A11564, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunoprecipitation - hnRNP A1 Rabbit mAb (A11564)}

Immunoprecipitation - hnRNP A1 Rabbit mAb (A11564)

Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg hnRNP A1 antibody (A11564). Western blot was performed from the immunoprecipitate using hnRNP A1 antibody (A11564) at a dilution of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (2)

Secondary Antibodies (26)