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hnRNP U Rabbit pAb (A3917)

Review (1)Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - hnRNP U Rabbit pAb (A3917)

Western blot analysis of various lysates using hnRNP U Rabbit pAb (A3917) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - hnRNP U Rabbit pAb (A3917)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using hnRNP U Rabbit pAb (A3917) at dilution of 1:50 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - hnRNP U Rabbit pAb (A3917)

Immunohistochemistry analysis of paraffin-embedded Rat testis using hnRNP U Rabbit pAb (A3917) at dilution of 1:50 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - hnRNP U Rabbit pAb (A3917)

Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using hnRNP U Rabbit pAb (A3917) at dilution of 1:50 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - hnRNP U Rabbit pAb (A3917)

Immunofluorescence analysis of C6 cells using hnRNP U Rabbit pAb (A3917) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - hnRNP U Rabbit pAb (A3917)

Immunoprecipitation analysis of 300 μg extracts of Jurkat cells using 3 μg hnRNP U antibody (A3917). Western blot was performed from the immunoprecipitate using hnRNP U antibody (A3917) at a dilution of 1:1000.

Review
ABclonal: review for hnRNP U Rabbit pAb(A3917)

Overview

Product namehnRNP U Rabbit pAb
Catalog No.A3917
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of a family of proteins that bind nucleic acids and function in the formation of ribonucleoprotein complexes in the nucleus with heterogeneous nuclear RNA (hnRNA). The encoded protein has affinity for both RNA and DNA, and binds scaffold-attached region (SAR) DNA. Mutations in this gene have been associated with epileptic encephalopathy, early infantile, 54. A pseudogene of this gene has been identified on chromosome 14.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 608-825 of human hnRNP U (NP_114032.2).
SequencePKDEDYKQRTQKKAEVEGKDLPEHAVLKMKGNFTLPEVAECFDEITYVELQKEEAQKLLEQYKEESKKALPPEKKQNTGSKKSNKNKSGKNQFNRGGGHRGRGGFNMRGGNFRGGAPGNRGGYNRRGNMPQRGGGGGGSGGIGYPYPRAPVFPGRGSYSNRGNYNRGGMPNRGNYNQNFRGRGNNRGYKNQSQGYNQWQQGQFWGQKPWSQHYHQGYY
Gene ID
Swiss Prot
SynonymsSAFA; DEE54; HNRPU; SAF-A; U21.1; pp120; EIEE54; GRIP120; hnRNP U; HNRNPU-AS1
Calculated MW91kDa
Observed MW120kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesA431, Jurkat, OVCAR3
Cellular locationCell surface, Cytoplasm, Nucleus
Customer validation

(293T)

WB(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - hnRNP U Rabbit pAb (A3917)}

Western blot - hnRNP U Rabbit pAb (A3917)

Western blot analysis of various lysates using hnRNP U Rabbit pAb (A3917) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - hnRNP U Rabbit pAb (A3917)}

Immunohistochemistry - hnRNP U Rabbit pAb (A3917)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using hnRNP U Rabbit pAb (A3917) at dilution of 1:50 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - hnRNP U Rabbit pAb (A3917)}

Immunohistochemistry - hnRNP U Rabbit pAb (A3917)

Immunohistochemistry analysis of paraffin-embedded Rat testis using hnRNP U Rabbit pAb (A3917) at dilution of 1:50 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - hnRNP U Rabbit pAb (A3917)}

Immunohistochemistry - hnRNP U Rabbit pAb (A3917)

Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using hnRNP U Rabbit pAb (A3917) at dilution of 1:50 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - hnRNP U Rabbit pAb (A3917)}

Immunofluorescence - hnRNP U Rabbit pAb (A3917)

Immunofluorescence analysis of C6 cells using hnRNP U Rabbit pAb (A3917) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - hnRNP U Rabbit pAb (A3917)}

Immunoprecipitation - hnRNP U Rabbit pAb (A3917)

Immunoprecipitation analysis of 300 μg extracts of Jurkat cells using 3 μg hnRNP U antibody (A3917). Western blot was performed from the immunoprecipitate using hnRNP U antibody (A3917) at a dilution of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

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