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p70 S6 Kinase 2 Rabbit mAb (A9100)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - p70 S6 Kinase 2 Rabbit mAb (A9100)

Western blot analysis of lysates from Mouse brain, using p70 S6 Kinase 2 Rabbit mAb (A9100) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunohistochemistry - p70 S6 Kinase 2 Rabbit mAb (A9100)

Immunohistochemistry analysis of p70 S6 Kinase 2 in paraffin-embedded human colon carcinoma tissue using p70 S6 Kinase 2 Rabbit mAb (A9100) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - p70 S6 Kinase 2 Rabbit mAb (A9100)

Immunohistochemistry analysis of p70 S6 Kinase 2 in paraffin-embedded rat colon tissue using p70 S6 Kinase 2 Rabbit mAb (A9100) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - p70 S6 Kinase 2 Rabbit mAb (A9100)

Immunohistochemistry analysis of p70 S6 Kinase 2 in paraffin-embedded mouse kidney tissue using p70 S6 Kinase 2 Rabbit mAb (A9100) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - p70 S6 Kinase 2 Rabbit mAb (A9100)

Immunohistochemistry analysis of p70 S6 Kinase 2 in paraffin-embedded human cervix cancer tissue using p70 S6 Kinase 2 Rabbit mAb (A9100) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - p70 S6 Kinase 2 Rabbit mAb (A9100)

Confocal imaging of C2C12 cells using p70 S6 Kinase 2 Rabbit mAb (A9100, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - p70 S6 Kinase 2 Rabbit mAb (A9100)

Confocal imaging of NIH/3T3 cells using p70 S6 Kinase 2 Rabbit mAb (A9100, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - p70 S6 Kinase 2 Rabbit mAb (A9100)

Confocal imaging of U-2 OS cells using p70 S6 Kinase 2 Rabbit mAb (A9100, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product namep70 S6 Kinase 2 Rabbit mAb
Catalog No.A9100
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1790
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains a kinase catalytic domain and phosphorylates the S6 ribosomal protein and eukaryotic translation initiation factor 4B (eIF4B). Phosphorylation of S6 leads to an increase in protein synthesis and cell proliferation.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human p70 S6 Kinase 2 (Q9UBS0).
SequenceMAAVFDLDLETEEGSEGEGEPELSPADACPLAELRAAGLEPVGHYEEVELTETSVNVGPERIGPHCFELLRVLGKGGYGKVFQVRKVQGTNLGKIYAMKVLRKAKIVRNAKDTAHTRAER
Gene ID
Swiss Prot
SynonymsKLS; SRK; S6K2; S6KB; STK14B; S6KI(2); S6Kbeta; p70S6Kb; P70-beta; S6K-beta2; P70-beta-1; P70-beta-2; p70(S6K)-beta; p70 S6 Kinase 2
Calculated MW53kDa
Observed MW60kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse brain
Cellular locationCytoplasm, Nucleus

Documents

Certificate of Compliance

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ABclonal:Western blot - p70 S6 Kinase 2 Rabbit mAb (A9100)}

Western blot - p70 S6 Kinase 2 Rabbit mAb (A9100)

Western blot analysis of lysates from Mouse brain, using p70 S6 Kinase 2 Rabbit mAb (A9100) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunohistochemistry - p70 S6 Kinase 2 Rabbit mAb (A9100)}

Immunohistochemistry - p70 S6 Kinase 2 Rabbit mAb (A9100)

Immunohistochemistry analysis of p70 S6 Kinase 2 in paraffin-embedded human colon carcinoma tissue using p70 S6 Kinase 2 Rabbit mAb (A9100) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - p70 S6 Kinase 2 Rabbit mAb (A9100)}

Immunohistochemistry - p70 S6 Kinase 2 Rabbit mAb (A9100)

Immunohistochemistry analysis of p70 S6 Kinase 2 in paraffin-embedded rat colon tissue using p70 S6 Kinase 2 Rabbit mAb (A9100) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - p70 S6 Kinase 2 Rabbit mAb (A9100)}

Immunohistochemistry - p70 S6 Kinase 2 Rabbit mAb (A9100)

Immunohistochemistry analysis of p70 S6 Kinase 2 in paraffin-embedded mouse kidney tissue using p70 S6 Kinase 2 Rabbit mAb (A9100) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - p70 S6 Kinase 2 Rabbit mAb (A9100)}

Immunohistochemistry - p70 S6 Kinase 2 Rabbit mAb (A9100)

Immunohistochemistry analysis of p70 S6 Kinase 2 in paraffin-embedded human cervix cancer tissue using p70 S6 Kinase 2 Rabbit mAb (A9100) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - p70 S6 Kinase 2 Rabbit mAb (A9100)}

Immunofluorescence - p70 S6 Kinase 2 Rabbit mAb (A9100)

Confocal imaging of C2C12 cells using p70 S6 Kinase 2 Rabbit mAb (A9100, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - p70 S6 Kinase 2 Rabbit mAb (A9100)}

Immunofluorescence - p70 S6 Kinase 2 Rabbit mAb (A9100)

Confocal imaging of NIH/3T3 cells using p70 S6 Kinase 2 Rabbit mAb (A9100, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - p70 S6 Kinase 2 Rabbit mAb (A9100)}

Immunofluorescence - p70 S6 Kinase 2 Rabbit mAb (A9100)

Confocal imaging of U-2 OS cells using p70 S6 Kinase 2 Rabbit mAb (A9100, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

* For research use only. Not for therapeutic or diagnostic purposes.

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