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pan 14-3-3 Rabbit mAb (A23829)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - pan 14-3-3 Rabbit mAb (A23829)

Western blot analysis of various lysates, using pan 14-3-3 Rabbit mAb (A23829) at 1:1500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - pan 14-3-3 Rabbit mAb (A23829)

Immunofluorescence analysis of H-4-11-E cells using pan 14-3-3 Rabbit mAb (A23829) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - pan 14-3-3 Rabbit mAb (A23829)

Immunofluorescence analysis of HeLa cells using pan 14-3-3 Rabbit mAb (A23829) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product namepan 14-3-3 Rabbit mAb
Catalog No.A23829
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC59297
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease.This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids human 1-100 of human pan 14-3-3 (NP_006817.1).
SequenceMEKTELIQKAKLAEQAERYDDMATCMKAVTEQGAELSNEERNLLSVAYKNVVGGRRSAWRVISSIEQKTDTSDKKLQLIKDYREKVESELRSICTTVLEL
Gene ID
Swiss Prot
Synonyms1C5; HS1; 14-3-3; GW128; YWHAA; KCIP-1; HEL-S-1; HEL4; YWHAD; HEL-S-3; POPCHAS; HEL-S-93; 14-3-3-zeta; pan 14-3-3
Calculated MW27kDa/28KDa/29KDa
Observed MW30kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1500 - 1:9000
  • IF/ICC 1:200 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesJurkat, HeLa, 293F, NIH/3T3, H-4-11-E
Cellular locationCytosol, Melanosome, Extracellular.

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - pan 14-3-3 Rabbit mAb (A23829)}

    Western blot - pan 14-3-3 Rabbit mAb (A23829)

    Western blot analysis of various lysates, using pan 14-3-3 Rabbit mAb (A23829) at 1:1500 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunofluorescence - pan 14-3-3 Rabbit mAb (A23829)}

    Immunofluorescence - pan 14-3-3 Rabbit mAb (A23829)

    Immunofluorescence analysis of H-4-11-E cells using pan 14-3-3 Rabbit mAb (A23829) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - pan 14-3-3 Rabbit mAb (A23829)}

    Immunofluorescence - pan 14-3-3 Rabbit mAb (A23829)

    Immunofluorescence analysis of HeLa cells using pan 14-3-3 Rabbit mAb (A23829) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)