제품 > 항체 > 다중클론항체(pAb)

perilipin-2 Rabbit pAb (A24464)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Rat

ABclonal:Western blot - perilipin-2 Rabbit pAb (A24464)

Western blot analysis of lysates from Mouse liver using perilipin-2 Rabbit pAb (A24464) at 1:5000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - perilipin-2 Rabbit pAb (A24464)

Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using perilipin-2 Rabbit pAb (A24464) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - perilipin-2 Rabbit pAb (A24464)

Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded rat liver using perilipin-2 Rabbit pAb (A24464) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameperilipin-2 Rabbit pAb
Catalog No.A24464
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Acts upstream of or within lipid storage and long-chain fatty acid transport. Located in several cellular components, including cytosol; lipid droplet; and nucleus. Is expressed in hindlimb tendon and pancreas epithelium. Orthologous to human PLIN2 (perilipin 2).
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 325-425 of mouse perilipin-2 (NP_031434.3).
SequenceTVLVNAQGLPQNIQDQAKHLGVMAGDIYSVFRNAASFKEVSDGVLTSSKGQLQKMKESLDEVMDYFVNNTPLNWLVGPFYPQSTEVNKASLKVQQSEVKAQ
Gene ID
Swiss Prot
SynonymsADPH; Adfp; Adrp; perilipin-2
Calculated MW46kDa
Observed MW52kDa
ReactivityRat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationImmunohistochemistry    Immunofluorescence    
Positive samplesMouse liver
Cellular locationMembrane.

Documents

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Lot number

    ABclonal:Western blot - perilipin-2 Rabbit pAb (A24464)}

    Western blot - perilipin-2 Rabbit pAb (A24464)

    Western blot analysis of lysates from Mouse liver using perilipin-2 Rabbit pAb (A24464) at 1:5000 dilution incubated overnight at 4℃.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunohistochemistry - perilipin-2 Rabbit pAb (A24464)}

    Immunohistochemistry - perilipin-2 Rabbit pAb (A24464)

    Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using perilipin-2 Rabbit pAb (A24464) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunofluorescence - perilipin-2 Rabbit pAb (A24464)}

    Immunofluorescence - perilipin-2 Rabbit pAb (A24464)

    Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded rat liver using perilipin-2 Rabbit pAb (A24464) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)