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phospho-LATS1-T1079+LATS2-T1041 Rabbit mAb (AP1517)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from HeLa cells using phospho-LATS1-T1079+LATS2-T1041 Rabbit mAb (AP1517) at 1:1000 dilution. HeLa cells were treated by Okadaic Acid (100 nM) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - phospho-LATS1-T1079+LATS2-T1041 Rabbit mAb (AP1517)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using phospho-LATS1-T1079+LATS2-T1041 Rabbit mAb (AP1517) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse intestin tissue using phospho-LATS1-T1079+LATS2-T1041 Rabbit mAb (AP1517) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using phospho-LATS1-T1079+LATS2-T1041 Rabbit mAb (AP1517) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using phospho-LATS1-T1079+LATS2-T1041 Rabbit mAb (AP1517) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using phospho-LATS1-T1079+LATS2-T1041 Rabbit mAb (AP1517) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue, Untreated (left) and lambda phosphatase-treated (right), using phospho-LATS1-T1079+LATS2-T1041 Rabbit mAb (AP1517) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue, Untreated (left) and lambda phosphatase-treated (right), using phospho-LATS1-T1079+LATS2-T1041 Rabbit mAb (AP1517) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat liver tissue, Untreated (left) and lambda phosphatase-treated (right), using phospho-LATS1-T1079+LATS2-T1041 Rabbit mAb (AP1517) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product name	phospho-LATS1-T1079+LATS2-T1041 Rabbit mAb
Catalog No.	AP1517
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC65149

Background

The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around T1079 and T1041 of human LATS1/ LATS2 (NP_004681.1).
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	LATS1; WARTS; wts; phospho-LATS1-T1079+LATS2-T1041
Calculated MW	76kDa/126kDa
Observed MW	140kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	HeLa treated by Okadaic Acid
Cellular location	Cytoplasm, centrosome, cytoskeleton, microtubule organizing center
Customer validation	WB(Other, Homo sapiens)