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AARS Rabbit pAb (A15017)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - AARS Rabbit pAb (A15017)

Western blot analysis of various lysates using AARS Rabbit pAb (A15017) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - AARS Rabbit pAb (A15017)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using AARS Rabbit pAb (A15017) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - AARS Rabbit pAb (A15017)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using AARS Rabbit pAb (A15017) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - AARS Rabbit pAb (A15017)

Immunohistochemistry analysis of paraffin-embedded Mouse liver using AARS Rabbit pAb (A15017) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - AARS Rabbit pAb (A15017)

Immunofluorescence analysis of 293T cells using AARS Rabbit pAb (A15017) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameAARS Rabbit pAb
Catalog No.A15017
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human AARS (NP_001596.2).
SequenceMDSTLTASEIRQRFIDFFKRNEHTYVHSSATIPLDDPTLLFANAGMNQFKPIFLNTIDPSHPMAKLSRAANTQKCIRAGGKHNDLDDVGKDVYHHTFFEM
Gene ID
Swiss Prot
SynonymsAARS; TTD8; CMT2N; DEE29; HDLS2; EIEE29
Calculated MW107kDa
Observed MW107kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, Jurkat, Mouse brain, Rat liver
Cellular locationCytoplasm.

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - AARS Rabbit pAb (A15017)}

    Western blot - AARS Rabbit pAb (A15017)

    Western blot analysis of various lysates using AARS Rabbit pAb (A15017) at 1:3000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunohistochemistry - AARS Rabbit pAb (A15017)}

    Immunohistochemistry - AARS Rabbit pAb (A15017)

    Immunohistochemistry analysis of paraffin-embedded Rat spleen using AARS Rabbit pAb (A15017) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunohistochemistry - AARS Rabbit pAb (A15017)}

    Immunohistochemistry - AARS Rabbit pAb (A15017)

    Immunohistochemistry analysis of paraffin-embedded Human lung cancer using AARS Rabbit pAb (A15017) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunohistochemistry - AARS Rabbit pAb (A15017)}

    Immunohistochemistry - AARS Rabbit pAb (A15017)

    Immunohistochemistry analysis of paraffin-embedded Mouse liver using AARS Rabbit pAb (A15017) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunofluorescence - AARS Rabbit pAb (A15017)}

    Immunofluorescence - AARS Rabbit pAb (A15017)

    Immunofluorescence analysis of 293T cells using AARS Rabbit pAb (A15017) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)