제품 > 항체 > 다중클론항체(pAb)

ABCA8A Rabbit pAb (A20496)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using ABCA8A Rabbit pAb (A20496) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - ABCA8A Rabbit pAb (A20496)

Immunohistochemistry analysis of paraffin-embedded Mouse lung using ABCA8A Rabbit pAb (A20496) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product name	ABCA8A Rabbit pAb
Catalog No.	A20496
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Predicted to enable ABC-type xenobiotic transporter activity and lipid transporter activity. Predicted to be involved in several processes, including cholesterol efflux; positive regulation of cholesterol efflux; and sphingomyelin biosynthetic process. Predicted to be located in endoplasmic reticulum and plasma membrane. Predicted to be active in intracellular membrane-bounded organelle. Is expressed in telencephalon. Orthologous to human ABCA8 (ATP binding cassette subfamily A member 8).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 690-828 of mouse ABCA8A (NP_694785.3).
Sequence	LKCAGSSLFLKKKWGIGYHLSLQLSETCVHERITSLVKQHIPDSKLSAESEGKLSYILPLERTNKFPDLYRDLERSPDLGIENYGVSITTLTEVFLKLEGKSSIDQSDIGMTEDVQAGGARSPERFAEVEQLVSLLNGR
Gene ID
Swiss Prot
Synonyms
Calculated MW	184kDa
Observed MW	180-250kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	Mouse heart, Rat lung, Rat liver
Cellular location	basolateral plasma membrane, endoplasmic reticulum, plasma membrane.
Customer validation	IF(Mus musculus)