제품 > 항체 > 단일클론항체(mAb)

ABflo® 450 Rabbit anti-Human CD116 mAb (A26805)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Flow cytometry: 1X10^6 293F cells (negative control, left) and Bewo cells (right) were surface-stained with ABflo® 450 Rabbit anti-Human CD116 mAb (A26805, 5 μl/Test, orange line) or ABflo® 450 Rabbit IgG isotype control (5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 Human PBMC cells were surface-stained with ABflo® 450 Rabbit anti-Human CD116 mAb (A26805, 5 μl/Test, orange line) or ABflo® 450 Rabbit IgG isotype control (5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line). Cells in the Monocytes gate were used for analysis.

Flow cytometry: 1X10^6 Bewo cells were surface-stained with ABflo® 450 Rabbit IgG isotype control (5 μl/Test, left) or ABflo® 450 Rabbit anti-Human CD116 mAb (A26805, 5 μl/Test, right).

Overview

Product name	ABflo® 450 Rabbit anti-Human CD116 mAb
Catalog No.	A26805
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC59275-ABflo450

Background

The protein encoded by this gene is the alpha subunit of the heterodimeric receptor for colony stimulating factor 2, a cytokine which controls the production, differentiation, and function of granulocytes and macrophages. The encoded protein is a member of the cytokine family of receptors. This gene is found in the pseudoautosomal region (PAR) of the X and Y chromosomes. Multiple transcript variants encoding different isoforms have been found for this gene, with some of the isoforms being membrane-bound and others being soluble.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 23-320 of human CD116 (NP_006131.2).
Sequence	EKSDLRTVAPASSLNVRFDSRTMNLSWDCQENTTFSKCFLTDKKNRVVEPRLSNNECSCTFREICLHEGVTFEVHVNTSQRGFQQKLLYPNSGREGTAAQNFSCFIYNADLMNCTWARGPTAPRDVQYFLYIRNSKRRREIRCPYYIQDSGTHVGCHLDNLSGLTSRNYFLVNGTSREIGIQFFDSLLDTKKIERFNPPSNVTVRCNTTHCLVRWKQPRTYQKLSYLDFQYQLDVHRKNTQPGTENLLINVSGDLENRYNFPSSEPRAKHSVKIRAADVRILNWSSWSEAIEFGSDDG
Gene ID
Swiss Prot
Synonyms	GMR; CD116; CSF2R; SMDP4; CDw116; CSF2RX; CSF2RY; GMCSFR; CSF2RAX; CSF2RAY; alphaGMR; GMR-alpha; GMCSFR-alpha; GM-CSF-R-alpha
Calculated MW	46kDa
Observed MW

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	plasma membrane, secreted, Cell membrane, Secreted, Single-pass type I membrane protein.

Documents

Certificate of Compliance

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Lot number

Research Area

ABflo® 450 Rabbit anti-Human CD116 mAb images

Flow CytoMetry - ABflo® 450 Rabbit anti-Human CD116 mAb (A26805)

Flow cytometry: 1X10^6 Bewo cells were surface-stained with ABflo® 450 Rabbit IgG isotype control (5 μl/Test, left) or ABflo® 450 Rabbit anti-Human CD116 mAb (A26805, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.