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CD116/GM-CSFRα Rabbit mAb (A23001)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - CD116/GM-CSFRα Rabbit mAb (A23001)

Western blot analysis of various lysates, using CD116/GM-CSFRα Rabbit mAb (A23001) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

Overview

Product nameCD116/GM-CSFRα Rabbit mAb
Catalog No.A23001
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC59284
The protein encoded by this gene is the alpha subunit of the heterodimeric receptor for colony stimulating factor 2, a cytokine which controls the production, differentiation, and function of granulocytes and macrophages. The encoded protein is a member of the cytokine family of receptors. This gene is found in the pseudoautosomal region (PAR) of the X and Y chromosomes. Multiple transcript variants encoding different isoforms have been found for this gene, with some of the isoforms being membrane-bound and others being soluble.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 23-320 of human CD116/GM-CSFRα (NP_006131.2).
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsGMR; CD116; CSF2R; SMDP4; CDw116; CSF2RX; CSF2RY; GMCSFR; CSF2RAX; CSF2RAY; alphaGMR; GMR-alpha; GMCSFR-alpha; GM-CSF-R-alpha; CD116/GM-CSFRα
Calculated MW27kDa/33kDa/38kDa/44kDa/46kDa/50kDa
Observed MW70-80kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesBewo
Cellular locationsecreted, Cell membrane, Secreted, Single-pass type I membrane protein

Documents

Certificate of Compliance

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ABclonal:Western blot - CD116/GM-CSFRα Rabbit mAb (A23001)}

Western blot - CD116/GM-CSFRα Rabbit mAb (A23001)

Western blot analysis of various lysates, using CD116/GM-CSFRα Rabbit mAb (A23001) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

* For research use only. Not for therapeutic or diagnostic purposes.

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