제품 > 항체 > 단일클론항체(mAb)

ABflo® 450 Rabbit anti-Human CD127/IL-7Rα mAb (A26070)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Flow cytometry: 1X10^6 293T cells (negative control, left) and Human PBMC (right) were surface-stained with ABflo® 450 Rabbit anti-Human CD127/IL-7Rα (A26070, 5 μl/Test, orange line) or ABflo® 450 Rabbit IgG isotype control (5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 Human PBMC were surface-stained with ABflo® 450 Rabbit IgG isotype control (5 μl/Test, left) or ABflo® 450 Rabbit anti-Human CD127/IL-7Rα (A26070, 5 μl/Test, right).

Review

Overview

Product name	ABflo® 450 Rabbit anti-Human CD127/IL-7Rα mAb
Catalog No.	A26070
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC66004-ABflo450

Background

The protein encoded by this gene is a receptor for interleukin 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukins 2, 4, 7, 9, and 15. This protein has been shown to play a critical role in V(D)J recombination during lymphocyte development. Defects in this gene may be associated with severe combined immunodeficiency (SCID). Alternatively spliced transcript variants have been found.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 21-236 human CD127/IL-7Rα (NP_002176.2).
Sequence	ESGYAQNGDLEDAELDDYSFSCYSQLEVNGSQHSLTCAFEDPDVNITNLEFEICGALVEVKCLNFRKLQEIYFIETKKFLLIGKSNICVKVGEKSLTCKKIDLTTIVKPEAPFDLSVVYREGANDFVVTFNTSHLQKKYVKVLMHDVAYRQEKDENKWTHVNLSSTKLTLLQRKLQPAAMYEIKVRSIPDHYFKGFWSEWSPSYYFRTPEINNSSG
Gene ID
Swiss Prot
Synonyms	ILRA; CD127; IL7RA; CDW127; IMD104; sIL-7R; lnc-IL7R; IL7Ralpha; IL-7Ralpha; IL-7R-alpha
Calculated MW	52kDa
Observed MW

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Cell membrane, Secreted, Single-pass type I membrane protein, Single-pass type I membrane protein.
Customer validation	(Human PBMCs)

Documents

Certificate of Compliance

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Lot number

Research Area

ABflo® 450 Rabbit anti-Human CD127/IL-7Rα mAb images

Flow CytoMetry - ABflo® 450 Rabbit anti-Human CD127/IL-7Rα mAb (A26070)

Flow cytometry: 1X10^6 Human PBMC were surface-stained with ABflo® 450 Rabbit IgG isotype control (5 μl/Test, left) or ABflo® 450 Rabbit anti-Human CD127/IL-7Rα (A26070, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.