제품 > 항체 > 단일클론항체(mAb)

ABflo® 450 Rabbit anti-Mouse CD304/Neuropilin-1 mAb (A26588)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Flow cytometry: 1X10^6 CHO cells (negative control, left) and CHO (Transfection, right) cells were surface-stained with ABflo® 450 Rabbit anti-Mouse CD304/Neuropilin-1 mAb (A26588, 5 μl/Test, orange line) or ABflo® 450 Rabbit IgG isotype control (5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 C57BL/6 mouse splenocytes were surface-stained with ABflo® 450 Rabbit IgG isotype control (5 μl/Test, left) or ABflo® 450 Rabbit anti-Mouse CD304/Neuropilin-1 mAb (A26588, 5 μl/Test, right).

Overview

Product name	ABflo® 450 Rabbit anti-Mouse CD304/Neuropilin-1 mAb
Catalog No.	A26588
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC69115-ABflo450

Background

Enables protein kinase binding activity; transmembrane signaling receptor activity; and vascular endothelial growth factor binding activity. Involved in several processes, including nervous system development; regulation of signal transduction; and vasculature development. Acts upstream of or within several processes, including morphogenesis of a branching epithelium; nervous system development; and toxin transport. Located in several cellular components, including endosome; focal adhesion; and neurofilament. Is integral component of postsynaptic membrane. Is expressed in several structures, including cardiovascular system; jaw; lung; nervous system; and sensory organ. Used to study retinal vein occlusion. Human ortholog(s) of this gene implicated in lung non-small cell carcinoma. Orthologous to human NRP1 (neuropilin 1).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 22-856 of mouse CD304/Neuropilin-1 (NP_032763.2).
Sequence	FRSDKCGGTIKIENPGYLTSPGYPHSYHPSEKCEWLIQAPEPYQRIMINFNPHFDLEDRDCKYDYVEVIDGENEGGRLWGKFCGKIAPSPVVSSGPFLFIKFVSDYETHGAGFSIRYEIFKRGPECSQNYTAPTGVIKSPGFPEKYPNSLECTYIIFAPKMSEIILEFESFDLEQDSNPPGGMFCRYDRLEIWDGFPEVGPHIGRYCGQKTPGRIRSSSGVLSMVFYTDSAIAKEGFSANYSVLQSSISEDFKCMEALGMESGEIHSDQITASSQYGTNWSVERSRLNYPENGWTPGEDSYKEWIQVDLGLLRFVTAVGTQGAISKETKKKYYVKTYRVDISSNGEDWISLKEGNKAIIFQGNTNPTDVVLGVFSKPLITRFVRIKPVSWETGISMRFEVYGCKITDYPCSGMLGMVSGLISDSQITASNQADRNWMPENIRLVTSRTGWALPPSPHPYTNEWLQVDLGDEKIVRGVIIQGGKHRENKVFMRKFKIAYSNNGSDWKTIMDDSKRKAKSFEGNNNYDTPELRTFSPLSTRFIRIYPERATHSGLGLRMELLGCEVEAPTAGPTTPNGNPVDECDDDQANCHSGTGDDFQLTGGTTVLATEKPTIIDSTIQSEFPTYGFNCEFGWGSHKTFCHWEHDSHAQLRWSVLTSKTGPIQDHTGDGNFIYSQADENQKGKVARLVSPVVYSQSSAHCMTFWYHMSGSHVGTLRVKLRYQKPEEYDQLVWMVVGHQGDHWKEGRVLLHKSLKLYQVIFEGEIGKGNLGGIAVDDISINNHISQEDCAKPTDLDKKNTEIKIDETGSTPGYEGEGEGDKNISRKPGNVLKTLDP
Gene ID
Swiss Prot
Synonyms	Nrp; NP-1; Npn1; NPN-1; C530029I03
Calculated MW	103kDa
Observed MW	Refer to figures

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Mitochondrion membrane, Single-pass type I membrane protein, Cell membrane, Cytoplasm.