제품 > 항체 > 단일클론항체(mAb)

CD304/Neuropilin-1 Rabbit PolymAb® (A26530PM)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using CD304/Neuropilin-1 Rabbit PolymAb® (A26530PM) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - CD304/Neuropilin-1 Rabbit PolymAb® (A26530PM)

Confocal imaging of Neuro-2a cells using CD304/Neuropilin-1 Rabbit PolymAb® (A26530PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

Confocal imaging of HUVEC cells using CD304/Neuropilin-1 Rabbit PolymAb® (A26530PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product name	CD304/Neuropilin-1 Rabbit PolymAb®
Catalog No.	A26530PM
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Enables protein kinase binding activity; transmembrane signaling receptor activity; and vascular endothelial growth factor binding activity. Involved in several processes, including nervous system development; regulation of signal transduction; and vasculature development. Acts upstream of or within several processes, including morphogenesis of a branching epithelium; nervous system development; and toxin transport. Located in several cellular components, including endosome; focal adhesion; and neurofilament. Is integral component of postsynaptic membrane. Is expressed in several structures, including cardiovascular system; jaw; lung; nervous system; and sensory organ. Used to study retinal vein occlusion. Human ortholog(s) of this gene implicated in lung non-small cell carcinoma. Orthologous to human NRP1 (neuropilin 1).

Immunogen information

Immunogen	Recombinant protein of mouse CD304/Neuropilin-1.
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	Nrp; NP-1; Npn1; NPN-1; C530029I03
Calculated MW	103kDa
Observed MW	130-140kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:2000 IF/ICC 1:200 - 1:800 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	HUVEC, Mouse brain, Rat brain
Cellular location	Mitochondrion membrane, Single-pass type I membrane protein, Cell membrane, Cytoplasm.