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ABflo® 488 Rabbit anti-Human CD263/TRAIL-R3 mAb (A24795)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD263/TRAIL-R3 mAb (A24795)

Flow cytometry: 1X10^6 K-562 cells (Low Expression, left) and BEWO cells (right) were surface-stained with ABflo® 488 Rabbit anti-Human CD263/TRAIL-R3 mAb (A24795, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD263/TRAIL-R3 mAb (A24795)

Flow cytometry: 1X10^6 BEWO cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human CD263/TRAIL-R3 mAb (A24795, 5 μl/Test, right).

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD263/TRAIL-R3 mAb (A24795)

Flow cytometry: 1X10^6 Human peripheral blood granulocytes were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human CD263/TRAIL-R3 mAb (A24795, 5 μl/Test, right).

Overview

Product nameABflo® 488 Rabbit anti-Human CD263/TRAIL-R3 mAb
Catalog No.A24795
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC63903-ABflo488
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains an extracellular TRAIL-binding domain and a transmembrane domain, but no cytoplasmic death domain. This receptor is not capable of inducing apoptosis, and is thought to function as an antagonistic receptor that protects cells from TRAIL-induced apoptosis. This gene was found to be a p53-regulated DNA damage-inducible gene. The expression of this gene was detected in many normal tissues but not in most cancer cell lines, which may explain the specific sensitivity of cancer cells to the apoptosis-inducing activity of TRAIL.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 26-235 of human CD263/TRAIL-R3(NP_003832.3).
SequenceATTARQEEVPQQTVAPQQQRHSFKGEECPAGSHRSEHTGACNPCTEGVDYTNASNNEPSCFPCTVCKSDQKHKSSCTMTRDTVCQCKEGTFRNENSPEMCRKCSRCPSGEVQVSNCTSWDDIQCVEEFGANATVETPAAEETMNTSPGTPAPAAEETMNTSPGTPAPAAEETMTTSPGTPAPAAEETMTTSPGTPAPAAEETMITSPGTP
Gene ID
Swiss Prot
SynonymsTNFRSF10C; CD263; DCR1; DCR1-TNFR; LIT; TRAIL-R3; TRAILR3; TRID; TNF receptor superfamily member 10c
Calculated MW27kDa
Observed MW
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationCell membrane, GPI-anchor, Lipid-anchor.

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ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD263/TRAIL-R3 mAb (A24795)}

Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD263/TRAIL-R3 mAb (A24795)

Flow cytometry: 1X10^6 K-562 cells (Low Expression, left) and BEWO cells (right) were surface-stained with ABflo® 488 Rabbit anti-Human CD263/TRAIL-R3 mAb (A24795, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD263/TRAIL-R3 mAb (A24795)}

Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD263/TRAIL-R3 mAb (A24795)

Flow cytometry: 1X10^6 BEWO cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human CD263/TRAIL-R3 mAb (A24795, 5 μl/Test, right).
ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD263/TRAIL-R3 mAb (A24795)}

Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD263/TRAIL-R3 mAb (A24795)

Flow cytometry: 1X10^6 Human peripheral blood granulocytes were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human CD263/TRAIL-R3 mAb (A24795, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.

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