제품 > 항체 > 단일클론항체(mAb)

ABflo® 488 Rabbit anti-Human ULBP2 mAb (A22694)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Flow cytometry:1X10^6 SH-SY5Y cells (negative control, Left) and HeLa cells (Right) were surface-stained with ABflo® 488 Rabbit anti-Human ULBP2 mAb(A22694, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry:1X10^6 HeLa cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human ULBP2 mAb(A22694, 5 μl/Test, right).

Overview

Product name	ABflo® 488 Rabbit anti-Human ULBP2 mAb
Catalog No.	A22694
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC54528-ABf488

Background

This gene encodes a major histocompatibility complex (MHC) class I-related molecule that binds to the NKG2D receptor on natural killer (NK) cells to trigger release of multiple cytokines and chemokines that in turn contribute to the recruitment and activation of NK cells. The encoded protein undergoes further processing to generate the mature protein that is either anchored to membrane via a glycosylphosphatidylinositol moiety, or secreted. Many malignant cells secrete the encoded protein to evade immunosurveillance by NK cells. This gene is located in a cluster of multiple MHC class I-related genes on chromosome 6.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 26-217 of human ULBP2 (NP_079493.1).
Sequence	GRADPHSLCYDITVIPKFRPGPRWCAVQGQVDEKTFLHYDCGNKTVTPVSPLGKKLNVTTAWKAQNPVLREVVDILTEQLRDIQLENYTPKEPLTLQARMSCEQKAEGHSSGSWQFSFDGQIFLLFDSEKRMWTTVHPGARKMKEKWENDKVVAMSFHYFSMGDCIGWLEDFLMGMDSTLEPSAGAPLAMSS
Gene ID
Swiss Prot
Synonyms	N2DL2; RAET1H; RAET1L; NKG2DL2; ALCAN-alpha
Calculated MW	27kDa
Observed MW

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Cell surface, endoplasmic reticulum, external side of plasma membrane, extracellular region, extracellular space, plasma membrane

Documents

Certificate of Compliance

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Research Area

ABflo® 488 Rabbit anti-Human ULBP2 mAb images

Flow CytoMetry - ABflo® 488 Rabbit anti-Human ULBP2 mAb (A22694)

Flow cytometry:1X10^6 HeLa cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human ULBP2 mAb(A22694, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.