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ULBP2 Rabbit mAb (A23828)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - ULBP2 Rabbit mAb (A23828)

Western blot analysis of various lysates, using ULBP2 Rabbit mAb (A23828) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

Overview

Product nameULBP2 Rabbit mAb
Catalog No.A23828
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC54529
This gene encodes a major histocompatibility complex (MHC) class I-related molecule that binds to the NKG2D receptor on natural killer (NK) cells to trigger release of multiple cytokines and chemokines that in turn contribute to the recruitment and activation of NK cells. The encoded protein undergoes further processing to generate the mature protein that is either anchored to membrane via a glycosylphosphatidylinositol moiety, or secreted. Many malignant cells secrete the encoded protein to evade immunosurveillance by NK cells. This gene is located in a cluster of multiple MHC class I-related genes on chromosome 6.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 26-217 of human ULBP2. (NP_079493.1).
SequenceGRADPHSLCYDITVIPKFRPGPRWCAVQGQVDEKTFLHYDCGNKTVTPVSPLGKKLNVTTAWKAQNPVLREVVDILTEQLRDIQLENYTPKEPLTLQARMSCEQKAEGHSSGSWQFSFDGQIFLLFDSEKRMWTTVHPGARKMKEKWENDKVVAMSFHYFSMGDCIGWLEDFLMGMDSTLEPSAGAPLAMSS
Gene ID
Swiss Prot
SynonymsULBP2; ALCAN-alpha; N2DL2; NKG2DL2; RAET1H; NKG2D ligand 2
Calculated MW27kDa
Observed MW30-35kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHCT116, U-87MG, Jurkat
Cellular locationCell membrane, GPI-anchor, Lipid-anchor, Secreted

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ABclonal:Western blot - ULBP2 Rabbit mAb (A23828)}

Western blot - ULBP2 Rabbit mAb (A23828)

Western blot analysis of various lysates, using ULBP2 Rabbit mAb (A23828) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (3)

재조합 단백질 (2)

Secondary Antibodies (26)