제품 > 항체 > 단일클론항체(mAb)

ABflo® 488 Rabbit anti-Mouse CD271/NGFR mAb (A24321)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Flow cytometry:1X10^6 293F cells (negative control, Left) and 293F(Transfection) cells (Right) were surface-stained with ABflo® 488 Rabbit anti-Mouse CD271/NGFR mAb(A24321, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry:1X10^6 293F(Transfection) cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Mouse CD271/NGFR mAb(A24321, 5 μl/Test, right)

Overview

Product name	ABflo® 488 Rabbit anti-Mouse CD271/NGFR mAb
Catalog No.	A24321
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC62149-ABflo488

Background

Enables death receptor activity; nerve growth factor binding activity; and small GTPase binding activity. Involved in several processes, including cellular glucose homeostasis; circadian regulation of gene expression; and dorsal aorta development. Acts upstream of or within several processes, including hair follicle morphogenesis; nervous system development; and regulation of signal transduction. Located in several cellular components, including cell surface; cell-cell junction; and postsynaptic density. Is expressed in several structures, including alimentary system; genitourinary system; integumental system; nervous system; and sensory organ. Human ortholog(s) of this gene implicated in Alzheimer's disease and mental depression. Orthologous to human NGFR (nerve growth factor receptor).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 32-254 of mouse CD271 (NP_150086.2) .
Sequence	KETCSTGMYTHSGECCKACNLGEGVAQPCGANQTVCEPCLDSVTFSDVVSATEPCKPCTECLGLQSMSAPCVEADDAVCRCSYGYYQDEETGRCEACSVCGVGSGLVFSCQDKQNTVCEECPEGTYSDEANHVDPCLPCTVCEDTERQLRECTPWADAECEEIPGRWITRSTPPEGSDVTTPSTQEPEAPPERDLIASTVADTVTTVMGSSQPVVTRGTADNL
Gene ID
Swiss Prot
Synonyms	p75; LNGFR; p75NTR; p75NGFR; Tnfrsf16
Calculated MW	45kDa
Observed MW	Refer to figures

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.03% proclin300, 0.2% BSA, 50% glycerol, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Cell membrane, Single-pass type I membrane protein, Curated Perikaryon, Cell projection, growth cone, Cell projection, dendritic spine