제품 > 항체 > 단일클론항체(mAb)

CD271 Rabbit mAb (A23913)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse, Rat

Western blot analysis of lysates from Mouse ovary, using CD271 Rabbit mAb (A23913) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Flow CytoMetry - CD271 Rabbit mAb (A23913)

Flow cytometry: 1X10^6 293F cells (negative control, Left) and 293F(Transfection) cells (Right) were surface-stained with CD271/NGFR Rabbit mAb(A23913, 2μg/mL, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 2μg/mL, blue line), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb(1:200 dilution) staining. Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry:1X10^6 293F(Transfection) cells cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 2 μg/mL, left) or CD271/NGFR Rabbit mAb(A23913, 2 μg/mL, right).

Overview

Product name	CD271 Rabbit mAb
Catalog No.	A23913
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC62149

Background

Enables death receptor activity; nerve growth factor binding activity; and small GTPase binding activity. Involved in several processes, including cellular glucose homeostasis; circadian regulation of gene expression; and dorsal aorta development. Acts upstream of or within several processes, including hair follicle morphogenesis; nervous system development; and regulation of signal transduction. Located in several cellular components, including cell surface; cell-cell junction; and postsynaptic density. Is expressed in several structures, including alimentary system; genitourinary system; integumental system; nervous system; and sensory organ. Human ortholog(s) of this gene implicated in Alzheimer's disease and mental depression. Orthologous to human NGFR (nerve growth factor receptor).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 32-254 of mouse CD271 (NP_150086.2) .
Sequence	KETCSTGMYTHSGECCKACNLGEGVAQPCGANQTVCEPCLDSVTFSDVVSATEPCKPCTECLGLQSMSAPCVEADDAVCRCSYGYYQDEETGRCEACSVCGVGSGLVFSCQDKQNTVCEECPEGTYSDEANHVDPCLPCTVCEDTERQLRECTPWADAECEEIPGRWITRSTPPEGSDVTTPSTQEPEAPPERDLIASTVADTVTTVMGSSQPVVTRGTADNL
Gene ID
Swiss Prot
Synonyms	p75; LNGFR; p75NTR; p75NGFR; Tnfrsf16; CD271
Calculated MW	45KDa
Observed MW	60-75kDa

Applications

Reactivity	Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:4000 FC 1:100 - 1:500 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Flow Cytometry
Positive samples	Mouse ovary
Cellular location	Cell membrane, Single-pass type I membrane protein, Curated Perikaryon, Cell projection, growth cone, Cell projection, dendritic spine.

Documents

Certificate of Compliance

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Research Area

CD271 Rabbit mAb images

Western blot - CD271 Rabbit mAb (A23913)

Flow CytoMetry - CD271 Rabbit mAb (A23913)

Flow cytometry:1X10^6 293F(Transfection) cells cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 2 μg/mL, left) or CD271/NGFR Rabbit mAb(A23913, 2 μg/mL, right).

* For research use only. Not for therapeutic or diagnostic purposes.