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ABflo® 488 Rabbit anti-Mouse CD56/NCAM mAb (A23369)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse CD56/NCAM mAb (A23369)

Flow cytometry:1X10^6 L-929 cells(negative control, left) and Neuro-2a cells (right) were surface-stained with ABflo® 488 Rabbit anti-Mouse CD56/NCAM mAb(A23369, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line).Non-fluorescently stained cells was used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse CD56/NCAM mAb (A23369)

Flow cytometry:1X10^6 Neuro-2a cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Mouse CD56/NCAM mAb(A23369, 5 μl/Test, right).

Overview

Product nameABflo® 488 Rabbit anti-Mouse CD56/NCAM mAb
Catalog No.A23369
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC60214-ABf488
Predicted to enable LRR domain binding activity and phosphatase binding activity. Involved in commissural neuron axon guidance and regulation of semaphorin-plexin signaling pathway. Acts upstream of or within several processes, including homotypic cell-cell adhesion; positive regulation of calcium-mediated signaling; and regulation of exocyst assembly. Located in several cellular components, including external side of plasma membrane; growth cone; and neuronal cell body. Is expressed in several structures, including embryo mesenchyme; nervous system; sensory organ; skin; and urinary system. Human ortholog(s) of this gene implicated in bipolar disorder; middle cerebral artery infarction; and pancreatic cancer. Orthologous to human NCAM1 (neural cell adhesion molecule 1).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 20-711 of mouse CD56 (NP_001106675.1).
SequenceLQVDIVPSQGEISVGESKFFLCQVAGDAKDKDISWFSPNGEKLSPNQQRISVVWNDDDSSTLTIYNANIDDAGIYKCVVTAEDGTQSEATVNVKIFQKLMFKNAPTPQEFKEGEDAVIVCDVVSSLPPTIIWKHKGRDVILKKDVRFIVLSNNYLQIRGIKKTDEGTYRCEGRILARGEINFKDIQVIVNVPPTVQARQSIVNATANLGQSVTLVCDADGFPEPTMSWTKDGEPIENEEEDDEKHIFSDDSSELTIRNVDKNDEAEYVCIAENKAGEQDASIHLKVFAKPKITYVENQTAMELEEQVTLTCEASGDPIPSITWRTSTRNISSEEKTLDGHMVVRSHARVSSLTLKSIQYTDAGEYICTASNTIGQDSQSMYLEFQYAPKLQGPVAVYTWEGNQVNITCEVFAYPSATISWFRDGQLLPSSNYSNIKIYNTPSASYLEVTPDSENDFGNYNCTAVNRIGQESLEFILVQADTPSSPSIDRVEPYSSTAQVQFDEPEATGGVPILKYKAEWKSLGEESWHFKWYDAKEANMEGIVTIMGLKPETRYSVRLAALNGKGLGEISAATEFKTQPVREPSAPKLEGQMGEDGNSIKVNLIKQDDGGSPIRHYLVKYRALASEWKPEIRLPSGSDHVMLKSLDWNAEYEVYVVAENQQGKSKAAHFVFRTSAQPTAIPANGSPTAGLST
Gene ID
Swiss Prot
SynonymsCD56; Ncam; E-NCAM; NCAM-1
Calculated MW67kDa/80kDa/93kDa/119kDa
Observed MWRefer to figures
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationCell membrane, Single-pass type I membrane protein

Documents

Certificate of Compliance

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Lot number

    ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse CD56/NCAM mAb (A23369)}

    Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse CD56/NCAM mAb (A23369)

    Flow cytometry:1X10^6 L-929 cells(negative control, left) and Neuro-2a cells (right) were surface-stained with ABflo® 488 Rabbit anti-Mouse CD56/NCAM mAb(A23369, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line).Non-fluorescently stained cells was used as blank control (red line).
    ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse CD56/NCAM mAb (A23369)}

    Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse CD56/NCAM mAb (A23369)

    Flow cytometry:1X10^6 Neuro-2a cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Mouse CD56/NCAM mAb(A23369, 5 μl/Test, right).

    * For research use only. Not for therapeutic or diagnostic purposes.

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    재조합 단백질 (1)

    Secondary Antibodies (26)