제품 > 항체 > 단일클론항체(mAb)

PE Rabbit anti-Mouse CD56/NCAM mAb (A23749)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Flow cytometry: 1X10^6 L-929 cells(negative control, left) and Neuro-2a cells (right) were surface-stained with PE Rabbit anti-Mouse CD56/NCAM mAb(A23749, 5 μl/Test, orange line) or PE Rabbit IgG isotype control (5 μl/Test, blue line).Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry:1X10^6 Neuro-2a cells were surface-stained with PE Rabbit IgG isotype control (5 μl/Test, left) or PE Rabbit anti-Mouse CD56/NCAM mAb(A23749, 5 μl/Test, right).

Overview

Product name	PE Rabbit anti-Mouse CD56/NCAM mAb
Catalog No.	A23749
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC60214-PE

Background

Predicted to enable LRR domain binding activity and phosphatase binding activity. Involved in commissural neuron axon guidance and regulation of semaphorin-plexin signaling pathway. Acts upstream of or within several processes, including homotypic cell-cell adhesion; positive regulation of calcium-mediated signaling; and regulation of exocyst assembly. Located in several cellular components, including external side of plasma membrane; growth cone; and neuronal cell body. Is expressed in several structures, including embryo mesenchyme; nervous system; sensory organ; skin; and urinary system. Human ortholog(s) of this gene implicated in bipolar disorder; middle cerebral artery infarction; and pancreatic cancer. Orthologous to human NCAM1 (neural cell adhesion molecule 1).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 20-711 of mouse CD56/NCAM (NP_001106675.1).
Sequence	LQVDIVPSQGEISVGESKFFLCQVAGDAKDKDISWFSPNGEKLSPNQQRISVVWNDDDSSTLTIYNANIDDAGIYKCVVTAEDGTQSEATVNVKIFQKLMFKNAPTPQEFKEGEDAVIVCDVVSSLPPTIIWKHKGRDVILKKDVRFIVLSNNYLQIRGIKKTDEGTYRCEGRILARGEINFKDIQVIVNVPPTVQARQSIVNATANLGQSVTLVCDADGFPEPTMSWTKDGEPIENEEEDDEKHIFSDDSSELTIRNVDKNDEAEYVCIAENKAGEQDASIHLKVFAKPKITYVENQTAMELEEQVTLTCEASGDPIPSITWRTSTRNISSEEKTLDGHMVVRSHARVSSLTLKSIQYTDAGEYICTASNTIGQDSQSMYLEFQYAPKLQGPVAVYTWEGNQVNITCEVFAYPSATISWFRDGQLLPSSNYSNIKIYNTPSASYLEVTPDSENDFGNYNCTAVNRIGQESLEFILVQADTPSSPSIDRVEPYSSTAQVQFDEPEATGGVPILKYKAEWKSLGEESWHFKWYDAKEANMEGIVTIMGLKPETRYSVRLAALNGKGLGEISAATEFKTQPVREPSAPKLEGQMGEDGNSIKVNLIKQDDGGSPIRHYLVKYRALASEWKPEIRLPSGSDHVMLKSLDWNAEYEVYVVAENQQGKSKAAHFVFRTSAQPTAIPANGSPTAGLST
Gene ID
Swiss Prot
Synonyms	CD56; Ncam; E-NCAM; NCAM-1
Calculated MW	67KDa/80KDa/93KDa/119KDa
Observed MW

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Plama membrane, extracellular, cytosol

Documents

Certificate of Compliance

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Lot number

Research Area

PE Rabbit anti-Mouse CD56/NCAM mAb images

Flow CytoMetry - PE Rabbit anti-Mouse CD56/NCAM mAb (A23749)

Flow cytometry:1X10^6 Neuro-2a cells were surface-stained with PE Rabbit IgG isotype control (5 μl/Test, left) or PE Rabbit anti-Mouse CD56/NCAM mAb(A23749, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.