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ABflo® 488 Rabbit anti-puromycin mAb (A23130)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Species independent

Flow cytometry:1X10^6 293T cells (negative control, Left) and 293T (treated with puromycin, right) cells were intracellularly-stained with ABflo® 488 Rabbit anti-puromycin mAb(A23130, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry:1X10^6 293T cells(treated with puromycin) were intracellularly-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 2 μg/mL, left) or ABflo® 488 Rabbit anti-puromycin mAb(A23130, 2 μg/mL, right).

Flow cytometry:1X10^6 RAW 264.7 cells (negative control, Left) and RAW 264.7(treated with puromycin, right) cells were intracellularly-stained with ABflo® 488 Rabbit anti-puromycin mAb(A23130, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry:1X10^6 Raw264.7 cells(treated with puromycin) cells were intracellularly-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-puromycin mAb(A23130, 5 μl/Test, right).

Flow cytometry:1X10^6 C6 cells (negative control, Left) and C6(treated with puromycin, right) cells were intracellularly-stained with ABflo® 488 Rabbit anti-puromycin mAb(A23130, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry:1X10^6 C6 cells(treated with puromycin) cells were intracellularly-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-puromycin mAb(A23130, 5 μl/Test, right).

Review

Overview

Product name	ABflo® 488 Rabbit anti-puromycin mAb
Catalog No.	A23130
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC58626-ABf488

Background

Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that causes premature chain termination during translation taking place in the ribosome. It has a role as a nucleoside antibiotic, an antiinfective agent, an antineoplastic agent, a protein synthesis inhibitor, an antimicrobial agent, an EC 3.4.11.14 (cytosol alanyl aminopeptidase) inhibitor and an EC 3.4.14.2 (dipeptidyl-peptidase II) inhibitor. It is a conjugate base of a puromycin(1+).Puromycin is an antibiotic that prevents bacterial protein translation. It is utilized as a selective agent in laboratory cell cultures. Puromycin is toxic to both prokaryotic and eukaryotic cells, resulting in significant cell death at appropriate doses.

Immunogen information

Immunogen	Chemical compounds corresponding to puromycin
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	Puromycin
Calculated MW
Observed MW

Applications

Reactivity	Species independent
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC (intra) 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application
Positive samples
Cellular location
Customer validation	(HEK293T cells)