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ABflo® 594 Rabbit anti-Human CD142 mAb (A24183)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD142 mAb (A24183)

Flow cytometry: 1X10^6 HEL cells (negative control, left) and A-431 cells (right) were surface-stained with ABflo® 594 Rabbit anti-Human CD142 mAb (A24183, 5 μl/Test, orange line) or ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD142 mAb (A24183)

Flow cytometry: 1X10^6 A-431 cells were surface-stained with ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, left) or ABflo® 594 Rabbit anti-Human CD142 mAb (A24183, 5 μl/Test, right).

Overview

Product nameABflo® 594 Rabbit anti-Human CD142 mAb
Catalog No.A24183
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC55325-ABflo594
This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces, for example, on monocytes. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. Platelets and monocytes have been shown to express this coagulation factor under procoagulatory and proinflammatory stimuli, and a major role in HIV-associated coagulopathy has been described. Platelet-dependent monocyte expression of coagulation factor III has been described to be associated with Coronavirus Disease 2019 (COVID-19) severity and mortality. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 34-251 of human CD142 (NP_001984.1).
SequenceGTTNTVAAYNLTWKSTNFKTILEWEPKPVNQVYTVQISTKSGDWKSKCFYTTDTECDLTDEIVKDVKQTYLARVFSYPAGNVESTGSAGEPLYENSPEFTPYLETNLGQPTIQSFEQVGTKVNVTVEDERTLVRRNNTFLSLRDVFGKDLIYTLYYWKSSSSGKKTAKTNTNEFLIDVDKGENYCFSVQAVIPSRTVNRKSTDSPVECMGQEKGEFRE
Gene ID
Swiss Prot
SynonymsTF; TFA; CD142
Calculated MW33kDa
Observed MW
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationCell surface, External side of plasma membrane, Extracellular space, Plasma membrane

Documents

Certificate of Compliance

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ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD142 mAb (A24183)}

Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD142 mAb (A24183)

Flow cytometry: 1X10^6 HEL cells (negative control, left) and A-431 cells (right) were surface-stained with ABflo® 594 Rabbit anti-Human CD142 mAb (A24183, 5 μl/Test, orange line) or ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD142 mAb (A24183)}

Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD142 mAb (A24183)

Flow cytometry: 1X10^6 A-431 cells were surface-stained with ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, left) or ABflo® 594 Rabbit anti-Human CD142 mAb (A24183, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.

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