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Coagulation factor III/Tissue Factor Rabbit mAb (A23243)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - Coagulation factor III/Tissue Factor Rabbit mAb (A23243)

Western blot analysis of various lysates, using Coagulation factor III/Tissue Factor Rabbit mAb (A23243) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - Coagulation factor III/Tissue Factor Rabbit mAb (A23243)

Immunohistochemistry analysis of paraffin-embedded Human brain tissue using Coagulation factor III/Tissue Factor Rabbit mAb (A23243) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Coagulation factor III/Tissue Factor Rabbit mAb (A23243)

Immunohistochemistry analysis of paraffin-embedded Human cervix tissue using Coagulation factor III/Tissue Factor Rabbit mAb (A23243) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Coagulation factor III/Tissue Factor Rabbit mAb (A23243)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Coagulation factor III/Tissue Factor Rabbit mAb (A23243) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Coagulation factor III/Tissue Factor Rabbit mAb (A23243)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using Coagulation factor III/Tissue Factor Rabbit mAb (A23243) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Coagulation factor III/Tissue Factor Rabbit mAb (A23243)

Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma tissue using Coagulation factor III/Tissue Factor Rabbit mAb (A23243) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Coagulation factor III/Tissue Factor Rabbit mAb (A23243)

Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue tissue using Coagulation factor III/Tissue Factor Rabbit mAb (A23243) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Coagulation factor III/Tissue Factor Rabbit mAb (A23243)

Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using Coagulation factor III/Tissue Factor Rabbit mAb (A23243) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - Coagulation factor III/Tissue Factor Rabbit mAb (A23243)

Immunofluorescence analysis of A-431 and MCF7(Negative sample) cells using Coagulation factor III/Tissue Factor Rabbit mAb (A23243) at dilution of 1:300 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameCoagulation factor III/Tissue Factor Rabbit mAb
Catalog No.A23243
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC60559
This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces, for example, on monocytes. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. Platelets and monocytes have been shown to express this coagulation factor under procoagulatory and proinflammatory stimuli, and a major role in HIV-associated coagulopathy has been described. Platelet-dependent monocyte expression of coagulation factor III has been described to be associated with Coronavirus Disease 2019 (COVID-19) severity and mortality. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 34-251 of human Coagulation factor III/Tissue Factor. (NP_001984.1).
SequenceGTTNTVAAYNLTWKSTNFKTILEWEPKPVNQVYTVQISTKSGDWKSKCFYTTDTECDLTDEIVKDVKQTYLARVFSYPAGNVESTGSAGEPLYENSPEFTPYLETNLGQPTIQSFEQVGTKVNVTVEDERTLVRRNNTFLSLRDVFGKDLIYTLYYWKSSSSGKKTAKTNTNEFLIDVDKGENYCFSVQAVIPSRTVNRKSTDSPVECMGQEKGEFRE
Gene ID
Swiss Prot
SynonymsTF; TFA; CD142; Coagulation factor III/Tissue Factor
Calculated MW33kDa
Observed MW45-53kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:100 - 1:500
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesBxPC-3, A-431, MDA-MB-231
Cellular locationMembrane, Secreted, Single-pass type I membrane protein.
Customer validation

WB(Cyprinus carpio)

Pull-down(Other)

Other(Other)

Documents

Certificate of Compliance

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ABclonal:Western blot - Coagulation factor III/Tissue Factor Rabbit mAb (A23243)}

Western blot - Coagulation factor III/Tissue Factor Rabbit mAb (A23243)

Western blot analysis of various lysates, using Coagulation factor III/Tissue Factor Rabbit mAb (A23243) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - Coagulation factor III/Tissue Factor Rabbit mAb (A23243)}

Immunohistochemistry - Coagulation factor III/Tissue Factor Rabbit mAb (A23243)

Immunohistochemistry analysis of paraffin-embedded Human brain tissue using Coagulation factor III/Tissue Factor Rabbit mAb (A23243) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Coagulation factor III/Tissue Factor Rabbit mAb (A23243)}

Immunohistochemistry - Coagulation factor III/Tissue Factor Rabbit mAb (A23243)

Immunohistochemistry analysis of paraffin-embedded Human cervix tissue using Coagulation factor III/Tissue Factor Rabbit mAb (A23243) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Coagulation factor III/Tissue Factor Rabbit mAb (A23243)}

Immunohistochemistry - Coagulation factor III/Tissue Factor Rabbit mAb (A23243)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Coagulation factor III/Tissue Factor Rabbit mAb (A23243) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Coagulation factor III/Tissue Factor Rabbit mAb (A23243)}

Immunohistochemistry - Coagulation factor III/Tissue Factor Rabbit mAb (A23243)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using Coagulation factor III/Tissue Factor Rabbit mAb (A23243) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Coagulation factor III/Tissue Factor Rabbit mAb (A23243)}

Immunohistochemistry - Coagulation factor III/Tissue Factor Rabbit mAb (A23243)

Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma tissue using Coagulation factor III/Tissue Factor Rabbit mAb (A23243) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Coagulation factor III/Tissue Factor Rabbit mAb (A23243)}

Immunohistochemistry - Coagulation factor III/Tissue Factor Rabbit mAb (A23243)

Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue tissue using Coagulation factor III/Tissue Factor Rabbit mAb (A23243) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Coagulation factor III/Tissue Factor Rabbit mAb (A23243)}

Immunohistochemistry - Coagulation factor III/Tissue Factor Rabbit mAb (A23243)

Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using Coagulation factor III/Tissue Factor Rabbit mAb (A23243) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunofluorescence - Coagulation factor III/Tissue Factor Rabbit mAb (A23243)}

Immunofluorescence - Coagulation factor III/Tissue Factor Rabbit mAb (A23243)

Immunofluorescence analysis of A-431 and MCF7(Negative sample) cells using Coagulation factor III/Tissue Factor Rabbit mAb (A23243) at dilution of 1:300 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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