제품 > 항체 > 단일클론항체(mAb)

ABflo® 594 Rabbit anti-Human CD273/PD-L2 mAb (A25275)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Flow cytometry: 1X10^6 Jurkat cells (negative control, left) and KARPAS 299 cells (right) were surface-stained with ABflo® 594 Rabbit anti-Human CD273/PD-L2 mAb (A25275, 5 μl/Test, orange line) or ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 KARPAS 299 cells were surface-stained with ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, left) or ABflo® 594 Rabbit anti-Human CD273/PD-L2 mAb (A25275, 5 μl/Test, right).

Overview

Product name	ABflo® 594 Rabbit anti-Human CD273/PD-L2 mAb
Catalog No.	A25275
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC65812-ABflo594

Background

Involved in negative regulation of activated T cell proliferation; negative regulation of interferon-gamma production; and negative regulation of interleukin-10 production. Predicted to be located in plasma membrane. Predicted to be active in external side of plasma membrane. Biomarker of pulmonary tuberculosis.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 20-219 of human CD273/PD-L2 (NP_079515.2).
Sequence	LFTVTVPKELYIIEHGSNVTLECNFDTGSHVNLGAITASLQKVENDTSPHRERATLLEEQLPLGKASFHIPQVQVRDEGQYQCIIIYGVAWDYKYLTLKVKASYRKINTHILKVPETDEVELTCQATGYPLAEVSWPNVSVPANTSHSRTPEGLYQVTSVLRLKPPPGRNFSCVFWNTHVRELTLASIDLQSQMEPRTHP
Gene ID
Swiss Prot
Synonyms	B7DC; Btdc; PDL2; CD273; PD-L2; PDCD1L2; bA574F11.2; PDCD1LG2
Calculated MW	21kDa/31kDa
Observed MW

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Cell membrane, Endomembrane system, Secreted