제품 > 항체 > 단일클론항체(mAb)

PD-L2/CD273 Rabbit mAb (A23287)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates, using PD-L2/CD273 Rabbit mAb (A23287) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - PD-L2/CD273 Rabbit mAb (A23287)

Immunohistochemistry analysis of paraffin-embedded mouse heart using PD-L2/CD273 Rabbit mAb (A23287) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded rat heart using PD-L2/CD273 Rabbit mAb (A23287) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product name	PD-L2/CD273 Rabbit mAb
Catalog No.	A23287
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC60190

Background

Involved in negative regulation of activated T cell proliferation; negative regulation of interferon-gamma production; and negative regulation of interleukin-10 production. Predicted to be located in plasma membrane. Predicted to be active in external side of plasma membrane. Biomarker of pulmonary tuberculosis.

Immunogen information

Immunogen	Recombinant protein of human PD-L2/CD273.
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	B7DC; Btdc; PDL2; CD273; PD-L2; PDCD1L2; bA574F11.2; PD-L2/CD273
Calculated MW	31kDa
Observed MW	45-60kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	HUVEC, Jurkat(Low expression control)
Cellular location	Cell membrane, Endomembrane system, Secreted