제품 > 항체 > 다중클론항체(pAb)

PDCD1LG2 Rabbit pAb (A17943)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of extracts of various cell lines, using PDCD1LG2 antibody (A17943) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - PDCD1LG2 Rabbit pAb (A17943)

Immunofluorescence analysis of HepG2 cells using PDCD1LG2 Rabbit pAb (A17943) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using PDCD1LG2 Rabbit pAb (A17943) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product name	PDCD1LG2 Rabbit pAb
Catalog No.	A17943
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Involved in negative regulation of activated T cell proliferation; negative regulation of interferon-gamma production; and negative regulation of interleukin-10 production. Predicted to be located in plasma membrane. Predicted to be active in external side of plasma membrane. Biomarker of pulmonary tuberculosis.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 100-200 of human PDCD1LG2 (NP_079515.2).
Sequence	YQCIIIYGVAWDYKYLTLKVKASYRKINTHILKVPETDEVELTCQATGYPLAEVSWPNVSVPANTSHSRTPEGLYQVTSVLRLKPPPGRNFSCVFWNTHVR
Gene ID
Swiss Prot
Synonyms	B7DC; Btdc; PDL2; CD273; PD-L2; PDCD1L2; bA574F11.2
Calculated MW	31kDa
Observed MW	45-60kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	Rat spleen, Rat thymus
Cellular location	external side of plasma membrane, extracellular region, plasma membrane
Customer validation	WB(Homo sapiens)