제품 > 항체 > 단일클론항체(mAb)

ABflo® 594 Rabbit anti-Human CD84 mAb (A24580)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Confocal imaging of HEL cells using ABflo® 594 Rabbit anti-Human CD84 mAb (A24580, dilution 1:200). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD84 mAb (A24580)

Flow cytometry: 1X10^6 HAP1 cells (negative control, left) and Raji cells (right) were surface-stained with ABflo® 594 Rabbit anti-Human CD84 mAb (A24580, 5 μl/Test, orange line) or ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 Raji cells were surface-stained with ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, left) or ABflo® 594 Rabbit anti-Human CD84 mAb (A24580, 5 μl/Test, right).

Overview

Product name	ABflo® 594 Rabbit anti-Human CD84 mAb
Catalog No.	A24580
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC63303-ABflo594

Background

This gene encodes a membrane glycoprotein that is a member of the signaling lymphocyte activation molecule (SLAM) family. This family forms a subset of the larger CD2 cell-surface receptor Ig superfamily. The encoded protein is a homophilic adhesion molecule that is expressed in numerous immune cells types and is involved in regulating receptor-mediated signaling in those cells. Alternate splicing results in multiple transcript variants.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 22-225 of human CD84 (NP_001171808.1).
Sequence	KDSEIFTVNGILGESVTFPVNIQEPRQVKIIAWTSKTSVAYVTPGDSETAPVVTVTHRNYYERIHALGPNYNLVISDLRMEDAGDYKADINTQADPYTTTKRYNLQIYRRLGKPKITQSLMASVNSTCNVTLTCSVEKEEKNVTYNWSPLGEEGNVLQIFQTPEDQELTYTCTAQNPVSNNSDSISARQLCADIAMGFRTHHTG
Gene ID
Swiss Prot
Synonyms	CD84; LY9B; SLAMF5; hCD84; mCD84; CD84 molecule
Calculated MW	24kDa/26kDa/30kDa/31kDa/36kDa/38kDa
Observed MW

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	IF/ICC 1:50 - 1:200 FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application	Immunofluorescence Flow Cytometry
Positive samples
Cellular location	Cell membrane, Single-pass type I membrane protein

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

ABflo® 594 Rabbit anti-Human CD84 mAb images

Immunofluorescence - ABflo® 594 Rabbit anti-Human CD84 mAb (A24580)

Confocal imaging of HEL cells using ABflo® 594 Rabbit anti-Human CD84 mAb (A24580, dilution 1:200). DAPI was used for nuclear staining (Blue). Objective: 100x.

Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD84 mAb (A24580)

Flow cytometry: 1X10^6 Raji cells were surface-stained with ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, left) or ABflo® 594 Rabbit anti-Human CD84 mAb (A24580, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.