제품 > 항체 > 단일클론항체(mAb)

CD84 Rabbit mAb (A24338)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of various lysates, using CD84 Rabbit mAb (A24338) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - CD84 Rabbit mAb (A24338)

Immunofluorescence analysis of HEL cells using CD84 Rabbit mAb (A24338) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Flow CytoMetry - CD84 Rabbit mAb (A24338)

Flow cytometry: 1X10^6 HAP1 cells (negative control, left) and Raji cells (right) were surface-stained with CD84 Rabbit mAb (A24338 , 2 μg/mL, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 Raji cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or CD84 Rabbit mAb (A24338 , 2 μg/mL, right).

Overview

Product name	CD84 Rabbit mAb
Catalog No.	A24338
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC63303

Background

This gene encodes a membrane glycoprotein that is a member of the signaling lymphocyte activation molecule (SLAM) family. This family forms a subset of the larger CD2 cell-surface receptor Ig superfamily. The encoded protein is a homophilic adhesion molecule that is expressed in numerous immune cells types and is involved in regulating receptor-mediated signaling in those cells. Alternate splicing results in multiple transcript variants.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 22-225 of human CD84 (NP_001171808.1).
Sequence	KDSEIFTVNGILGESVTFPVNIQEPRQVKIIAWTSKTSVAYVTPGDSETAPVVTVTHRNYYERIHALGPNYNLVISDLRMEDAGDYKADINTQADPYTTTKRYNLQIYRRLGKPKITQSLMASVNSTCNVTLTCSVEKEEKNVTYNWSPLGEEGNVLQIFQTPEDQELTYTCTAQNPVSNNSDSISARQLCADIAMGFRTHHTG
Gene ID
Swiss Prot
Synonyms	LY9B; hCD84; mCD84; SLAMF5; CD84
Calculated MW	39kDa
Observed MW	64-82kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200 FC 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence Flow Cytometry
Positive samples	Raji
Cellular location	Cell membrane, Single-pass type I membrane protein

Documents

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Research Area

CD84 Rabbit mAb images

Western blot - CD84 Rabbit mAb (A24338)

Immunofluorescence - CD84 Rabbit mAb (A24338)

Flow CytoMetry - CD84 Rabbit mAb (A24338)

Flow cytometry: 1X10^6 Raji cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or CD84 Rabbit mAb (A24338 , 2 μg/mL, right).

* For research use only. Not for therapeutic or diagnostic purposes.