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ABflo® 594 Rabbit anti-Human Flt3/CD135  mAb (A24382)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Human Flt3/CD135  mAb (A24382)

Flow cytometry:1X10^6 HEL cells (negative control, Left) and REH cells(Right) were surface-stained with ABflo® 594 Rabbit anti-Human Flt3/CD135 mAb(A24382, 5 μl/Test, orange line) or ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Human Flt3/CD135  mAb (A24382)

Flow cytometry:1X10^6 REH cells were surface-stained with ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, left) or ABflo® 594 Rabbit anti-Human Flt3/CD135 mAb(A24382, 5 μl/Test, right).

Overview

Product nameABflo® 594 Rabbit anti-Human Flt3/CD135  mAb
Catalog No.A24382
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC63039-ABflo594
This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. This receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 27-163 of human Flt3/CD135 (NP_004110.2).
SequenceNQDLPVIKCVLINHKNNDSSVGKSSSYPMVSESPEDLGCALRPQSSGTVYEAAAVEVDVSASITLQVLVDAPGNISCLWVFKHSSLNCQPHFDLQNRGVVSMVILKMTETQAGEYLLFIQSEATNYTILFTVSIRNT
Gene ID
Swiss Prot
SynonymsFLT3; CD135; FLK-2; FLK2; STK1; fms related tyrosine kinase 3
Calculated MW108kDa/112kDa
Observed MW
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationEndoplasmic reticulum lumen, Membrane, Single-pass type I membrane protein

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ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Human Flt3/CD135  mAb (A24382)}

Flow CytoMetry - ABflo® 594 Rabbit anti-Human Flt3/CD135  mAb (A24382)

Flow cytometry:1X10^6 HEL cells (negative control, Left) and REH cells(Right) were surface-stained with ABflo® 594 Rabbit anti-Human Flt3/CD135 mAb(A24382, 5 μl/Test, orange line) or ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Human Flt3/CD135  mAb (A24382)}

Flow CytoMetry - ABflo® 594 Rabbit anti-Human Flt3/CD135  mAb (A24382)

Flow cytometry:1X10^6 REH cells were surface-stained with ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, left) or ABflo® 594 Rabbit anti-Human Flt3/CD135 mAb(A24382, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.

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