제품 > 항체 > 단일클론항체(mAb)

ABflo® 594 Rabbit anti-Human JAM-A/CD321/F11R mAb (A24199)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Flow cytometry: 1X10^6 U-2 OS cells (negative control, left) and HT-29 cells (right) were surface-stained with ABflo® 594 Rabbit anti-Human JAM-A/CD321/F11R mAb (A24199, 5 μl/Test, orange line) or ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 HT-29 cells were surface-stained with ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, left) or ABflo® 594 Rabbit anti-Human JAM-A/CD321/F11R mAb (A24199, 5 μl/Test, right).

Overview

Product name	ABflo® 594 Rabbit anti-Human JAM-A/CD321/F11R mAb
Catalog No.	A24199
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC53966-ABflo594

Background

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor. Multiple 5' alternatively spliced variants, encoding the same protein, have been identified but their biological validity has not been established.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 28-242 of human JAM-A/CD321/F11R (NP_058642.1).
Sequence	SVTVHSSEPEVRIPENNPVKLSCAYSGFSSPRVEWKFDQGDTTRLVCYNNKITASYEDRVTFLPTGITFKSVTREDTGTYTCMVSEEGGNSYGEVKVKLIVLVPPSKPTVNIPSSATIGNRAVLTCSEQDGSPPSEYTWFKDGIVMPTNPKSTRAFSNSSYVLNPTTGELVFDPLSASDTGEYSCEARNGYGTPMTSNAVRMEAVERNVGVIVAA
Gene ID
Swiss Prot
Synonyms	JAM; KAT; JAM1; JAMA; JCAM; CD321; PAM-1
Calculated MW	27kDa/32kDa
Observed MW

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Bicellular tight junction, cell junction, cell-cell junction, extracellular exosome, plasma membrane, tight junction