제품 > 항체 > 다중클론항체(pAb)

JAM-A/CD321/F11R Rabbit pAb (A1241)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using JAM-A/CD321/F11R Rabbit pAb (A1241) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - JAM-A/CD321/F11R Rabbit pAb (A1241)

Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using JAM-A/CD321/F11R Rabbit pAb (A1241) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human placenta using JAM-A/CD321/F11R Rabbit pAb (A1241) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product name	JAM-A/CD321/F11R Rabbit pAb
Catalog No.	A1241
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor. Multiple 5' alternatively spliced variants, encoding the same protein, have been identified but their biological validity has not been established.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 28-238 of human JAM-A/CD321/F11R (NP_058642.1).
Sequence	SVTVHSSEPEVRIPENNPVKLSCAYSGFSSPRVEWKFDQGDTTRLVCYNNKITASYEDRVTFLPTGITFKSVTREDTGTYTCMVSEEGGNSYGEVKVKLIVLVPPSKPTVNIPSSATIGNRAVLTCSEQDGSPPSEYTWFKDGIVMPTNPKSTRAFSNSSYVLNPTTGELVFDPLSASDTGEYSCEARNGYGTPMTSNAVRMEAVERNVGV
Gene ID
Swiss Prot
Synonyms	JAM; KAT; JAM1; JAMA; JCAM; CD321; PAM-1; JAM-A/CD321/F11R
Calculated MW	33kDa
Observed MW	37kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	HepG2, SKOV3, Mouse lung, Rat lung
Cellular location	Cell junction, Cell membrane, Single-pass type I membrane protein, tight junction.
Customer validation	WB(Sus scrofa, Mus musculus, Homo sapiens, Mus musculus, Gallus gallus) IF(Mus musculus) Other(Gallus gallus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

JAM-A/CD321/F11R Rabbit pAb images

Western blot - JAM-A/CD321/F11R Rabbit pAb (A1241)

Immunohistochemistry - JAM-A/CD321/F11R Rabbit pAb (A1241)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for JAM-A/CD321/F11R Rabbit pAb (A1241)

Product:

JAM-A/CD321/F11R Rabbit pAb

Journal:

Frontiers in microbiology

Application:

IF:

6.1

Species:

Sus scrofa

PMID:

29867808

Title:

Lactobacillus frumenti Facilitates Intestinal Epithelial Barrier Function Maintenance in Early-Weaned Piglets

References for JAM-A/CD321/F11R Rabbit pAb (A1241)

Product:

JAM-A/CD321/F11R Rabbit pAb

Journal:

Food & function

Application:

IF:

5.1

Species:

Mus musculus

PMID:

31187839

Title:

Red yeast rice ameliorates high-fat diet-induced atherosclerosis in Apoe-/- mice in association with improved inflammation and altered gut microbiota composition.

References for JAM-A/CD321/F11R Rabbit pAb (A1241)

Product:

JAM-A/CD321/F11R Rabbit pAb

Journal:

International journal of environmental research and public health

Application:

IF:

Species:

Homo sapiens

PMID:

36767519

Title:

Impact of Plasticizer on the Intestinal Epithelial Integrity and Tissue-Repairing Ability within Cells in the Proximity of the Human Gut Microbiome - PMC

References for JAM-A/CD321/F11R Rabbit pAb (A1241)

Product:

JAM-A/CD321/F11R Rabbit pAb

Journal:

Current pharmaceutical design

Application:

IF:

2.6

Species:

Mus musculus

PMID:

36415092

Title:

Mechanism of HSP90 Inhibitor in the Treatment of DSS-induced Colitis in Mice by Inhibiting MAPK Pathway and Synergistic Effect of Compound Sophora Decoction

References for JAM-A/CD321/F11R Rabbit pAb (A1241)

Product:

JAM-A/CD321/F11R Rabbit pAb

Journal:

Cell Proliferation

Application:

IF:

8.5

Species:

Homo sapiens, Mus musculus

PMID:

31418947

Title:

Inhibition of CREB-mediated ZO-1 and Activation of NF-B-induced IL-6 by Colonic Epithelial MCT4 Destroys Intestinal Barrier Function.

References for JAM-A/CD321/F11R Rabbit pAb (A1241)

Product:

JAM-A/CD321/F11R Rabbit pAb

Journal:

Poultry science

Application:

IF:

4.4

Species:

Gallus gallus

PMID:

38593549

Title:

Zinc alleviates thermal stress-induced damage to the integrity and barrier function of cultured chicken embryonic primary jejunal epithelial cells via the MAPK and PI3K/AKT/mTOR signaling pathways - PMC

References for JAM-A/CD321/F11R Rabbit pAb (A1241)

Product:

JAM-A/CD321/F11R Rabbit pAb

Journal:

Journal of animal science and biotechnology

Application:

WB, Other

IF:

6.3

Species:

Gallus gallus

PMID:

39217350

Title:

Dietary Zn proteinate with moderate chelation strength alleviates heat stress-induced intestinal barrier function damage by promoting expression of tight junction proteins via the A20/NF-B p65/MMP-2 pathway in the jejunum of broilers - PMC