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ABflo® 594 Rabbit anti-Mouse CD11a mAb (A25829)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Mouse CD11a mAb (A25829)

Flow cytometry: 1X10^6 NIH/3T3 cells (Low Expression, left) and C57BL/6 mouse splenocytes (right) were surface-stained with ABflo® 594 Rabbit anti-Mouse CD11a mAb (A25829, 5 μl/Test, orange line) or ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Mouse CD11a mAb (A25829)

Flow cytometry: 1X10^6 C57BL/6 mouse splenocytes were surface-stained with ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, left) or ABflo® 594 Rabbit anti-Mouse CD11a mAb (A25829, 5 μl/Test, right).

Overview

Product nameABflo® 594 Rabbit anti-Mouse CD11a mAb
Catalog No.A25829
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC67614-ABflo594
Predicted to enable ICAM-3 receptor activity and cell adhesion molecule binding activity. Involved in cell-cell adhesion. Acts upstream of or within several processes, including activated T cell proliferation; positive regulation of T cell proliferation; and positive regulation of calcium-mediated signaling. Located in cell-cell junction; external side of plasma membrane; and immunological synapse. Is expressed in heart; hemolymphoid system; and vault of skull. Orthologous to human ITGAL (integrin subunit alpha L).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 149-340 of mouse CD11a (NP_001240801.1).
SequenceKGKVDLVFLFDGSQSLDRKDFEKILEFMKDVMRKLSNTSYQFAAVQFSTDCRTEFTFLDYVKQNKNPDVLLGSVQPMFLLTNTFRAINYVVAHVFKEESGARPDATKVLVIITDGEASDKGNISAAHDITRYIIGIGKHFVSVQKQKTLHIFASEPVEEFVKILDTFEKLKDLFTDLQRRIYAIEGTNRQDL
Gene ID
Swiss Prot
SynonymsCd11a; LFA-1; Ly-15; Ly-21; (p180); LFA-1A
Calculated MW128kDa
Observed MW
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationCell membrane; Single-pass type I membrane protein

Documents

Certificate of Compliance

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    ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Mouse CD11a mAb (A25829)}

    Flow CytoMetry - ABflo® 594 Rabbit anti-Mouse CD11a mAb (A25829)

    Flow cytometry: 1X10^6 NIH/3T3 cells (Low Expression, left) and C57BL/6 mouse splenocytes (right) were surface-stained with ABflo® 594 Rabbit anti-Mouse CD11a mAb (A25829, 5 μl/Test, orange line) or ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Mouse CD11a mAb (A25829)}

    Flow CytoMetry - ABflo® 594 Rabbit anti-Mouse CD11a mAb (A25829)

    Flow cytometry: 1X10^6 C57BL/6 mouse splenocytes were surface-stained with ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, left) or ABflo® 594 Rabbit anti-Mouse CD11a mAb (A25829, 5 μl/Test, right).

    * For research use only. Not for therapeutic or diagnostic purposes.

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