제품 > 항체 > 단일클론항체(mAb)

ABflo® 594 Rabbit anti-Mouse CD68 mAb (A23740)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Confocal imaging of RAW 264.7 cells using ABflo® 594 Rabbit anti-Mouse CD68 mAb (A23740, dilution 1:25). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Mouse CD68 mAb (A23740)

Flow cytometry: 1X10^6 NIH/3T3 cells(negative control, left) and Raw264.7 cells (right) were surface-stained with ABflo® 594 Rabbit anti-Mouse CD68 mAb(A23740, 5 μl/Test, orange line) or ABflo® 594 Rabbit IgG isotype control (5 μl/Test, blue line).Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry:1X10^6 Raw264.7 cells were surface-stained with ABflo® 594 Rabbit IgG isotype control (5 μl/Test, left) or ABflo® 594 Rabbit anti-Mouse CD68 mAb(A23740, 5 μl/Test, right).

Overview

Product name	ABflo® 594 Rabbit anti-Mouse CD68 mAb
Catalog No.	A23740
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC60987-ABf594

Background

Involved in several processes, including cellular response to lipopolysaccharide; cellular response to oxidised low-density lipoprotein particle stimulus; and negative regulation of dendritic cell antigen processing and presentation. Acts upstream of or within aging and cellular response to organic substance. Located in lysosome and plasma membrane. Is expressed in several structures, including central nervous system; embryo mesenchyme; heart blood vessel; liver; and spleen. Orthologous to human CD68 (CD68 molecule).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 21-291 of mouse CD68 (NP_001277987.1).
Sequence	DCPHKKAVTLLPSFTMTPTATESTASPTTSHRPTTTSHGNVTVHTSSGPTTVTHNPATTTSHGNATISHATVSPTTNGTATSPRSSTVGPHPGPPPPSPSPRSKGALGNYTWANGSQPCVQLQAQIQIRILYPIQGGRKAWGISVLNPNKTKVQGGCDGTHPHLSLSFPYGQLTFGFKQDLHQSPSTVYLDYMAVEYNVSFPQAAQWTFMAQNSSLRELQAPLGQSFCCGNASIVLSPAVHLDLLSLRLQAAQLPDKGHFGPCFSCNRDQS
Gene ID
Swiss Prot
Synonyms	Lamp4; gp110; Scard1
Calculated MW	33KDa/34KDa
Observed MW

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	IF/ICC 1:50 - 1:200 FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application	Immunofluorescence Flow Cytometry
Positive samples
Cellular location	Lysosome, plasma membrane

Documents

Certificate of Compliance

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Research Area

ABflo® 594 Rabbit anti-Mouse CD68 mAb images

Immunofluorescence - ABflo® 594 Rabbit anti-Mouse CD68 mAb (A23740)

Confocal imaging of RAW 264.7 cells using ABflo® 594 Rabbit anti-Mouse CD68 mAb (A23740, dilution 1:25). DAPI was used for nuclear staining (Blue). Objective: 100x.

Flow CytoMetry - ABflo® 594 Rabbit anti-Mouse CD68 mAb (A23740)

Flow cytometry:1X10^6 Raw264.7 cells were surface-stained with ABflo® 594 Rabbit IgG isotype control (5 μl/Test, left) or ABflo® 594 Rabbit anti-Mouse CD68 mAb(A23740, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.