제품 > 항체 > 단일클론항체(mAb)

CD68 Rabbit mAb (A23534)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Western blot analysis of various lysates, using CD68 Rabbit mAb (A23534) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - CD68 Rabbit mAb (A23534)

Immunofluorescence analysis of RAW264.7 cells using CD68 Rabbit mAb (A23534) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Flow CytoMetry - CD68 Rabbit mAb (A23534)

Flow cytometry: 1X10^6 NIH/3T3 cells (negative control, left) and RAW 264.7 cells (right) were surface-stained with CD68 Rabbit mAb (A23534, 2 μg/mL, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 RAW 264.7 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or CD68 Rabbit mAb (A23534, 2 μg/mL, right).

Overview

Product name	CD68 Rabbit mAb
Catalog No.	A23534
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC60987

Background

Involved in several processes, including cellular response to lipopolysaccharide; cellular response to oxidised low-density lipoprotein particle stimulus; and negative regulation of dendritic cell antigen processing and presentation. Acts upstream of or within aging and cellular response to organic substance. Located in lysosome and plasma membrane. Is expressed in several structures, including central nervous system; embryo mesenchyme; heart blood vessel; liver; and spleen. Orthologous to human CD68 (CD68 molecule).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 21–291 of mouse CD68 (NP_001277987.1).
Sequence	DCPHKKAVTLLPSFTMTPTATESTASPTTSHRPTTTSHGNVTVHTSSGPTTVTHNPATTTSHGNATISHATVSPTTNGTATSPRSSTVGPHPGPPPPSPSPRSKGALGNYTWANGSQPCVQLQAQIQIRILYPIQGGRKAWGISVLNPNKTKVQGGCDGTHPHLSLSFPYGQLTFGFKQDLHQSPSTVYLDYMAVEYNVSFPQAAQWTFMAQNSSLRELQAPLGQSFCCGNASIVLSPAVHLDLLSLRLQAAQLPDKGHFGPCFSCNRDQS
Gene ID
Swiss Prot
Synonyms	Lamp4; gp110; Scard1; CD68
Calculated MW	33KDa/34KDa
Observed MW	70-80kDa, 130-140kDa

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200 FC 1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence Flow Cytometry
Positive samples	RAW264.7
Cellular location
Customer validation	IHC(Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

CD68 Rabbit mAb images

Western blot - CD68 Rabbit mAb (A23534)

Immunofluorescence - CD68 Rabbit mAb (A23534)

Flow CytoMetry - CD68 Rabbit mAb (A23534)

Flow cytometry: 1X10^6 RAW 264.7 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or CD68 Rabbit mAb (A23534, 2 μg/mL, right).

* For research use only. Not for therapeutic or diagnostic purposes.