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ABflo® 647 Conjugated AIF1/IBA1 Rabbit mAb (A26033)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ABflo® 647 Conjugated AIF1/IBA1 Rabbit mAb (A26033)

Western blot analysis of lysates from THP-1 cells using ABflo® 647 Conjugated AIF1/IBA1 Rabbit mAb (A26033) at 1:5000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - ABflo® 647 Conjugated AIF1/IBA1 Rabbit mAb (A26033)

Western blot analysis of various lysates using ABflo® 647 Conjugated AIF1/IBA1 Rabbit mAb (A26033) at 1:5000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - ABflo® 647 Conjugated AIF1/IBA1 Rabbit mAb (A26033)

Confocal imaging of paraffin-embedded Human brain tissue using ABflo® 647 Conjugated AIF1/IBA1 Rabbit mAb (A26033, dilution 1:500). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.

ABclonal:Immunofluorescence - ABflo® 647 Conjugated AIF1/IBA1 Rabbit mAb (A26033)

Confocal imaging of paraffin-embedded Rat brain tissue using ABflo® 647 Conjugated AIF1/IBA1 Rabbit mAb (A26033, dilution 1:500). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.

ABclonal:Immunofluorescence - ABflo® 647 Conjugated AIF1/IBA1 Rabbit mAb (A26033)

Confocal imaging of paraffin-embedded Mouse brain tissue using ABflo® 647 Conjugated AIF1/IBA1 Rabbit mAb (A26033, dilution 1:500). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.

Overview

Product nameABflo® 647 Conjugated AIF1/IBA1 Rabbit mAb
Catalog No.A26033
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2301-ABf647
Enables actin filament binding activity and calcium ion binding activity. Involved in several processes, including Rac protein signal transduction; actin filament organization; and ruffle assembly. Acts upstream of or within actin filament bundle assembly. Located in several cellular components, including actin filament; phagocytic cup; and ruffle membrane. Is expressed in adrenal cortex; central nervous system; embryo mesenchyme; and retina. Human ortholog(s) of this gene implicated in type 1 diabetes mellitus. Orthologous to human AIF1 (allograft inflammatory factor 1).
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of mouse AIF1/IBA1 (O70200).
SequenceMSQSRDLQGGKAFGLLKAQQEERLEGINKQFLDDPKYSNDEDLPSKLEAFKVKYMEFDLNGNGDIDIMSLKRMLEKLGVPKTHLELKRLIREVSSGSEET
Gene ID
Swiss Prot
SynonymsG1; Iba1; AIF-1; D17H6S50E
Calculated MW17kDa
Observed MW17kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2500 - 1:10000
  • IF/ICC 1:200 - 1:1000
Storage bufferStore at -20℃. Avoid freeze.
Buffer:PBS with 0.03% proclin300, 0.2% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse testis, Mouse spleen, THP-1
Cellular locationCell projection, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, cytoskeleton, ruffle membrane.

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - ABflo® 647 Conjugated AIF1/IBA1 Rabbit mAb (A26033)}

    Western blot - ABflo® 647 Conjugated AIF1/IBA1 Rabbit mAb (A26033)

    Western blot analysis of lysates from THP-1 cells using ABflo® 647 Conjugated AIF1/IBA1 Rabbit mAb (A26033) at 1:5000 dilution incubated overnight at 4℃.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Western blot - ABflo® 647 Conjugated AIF1/IBA1 Rabbit mAb (A26033)}

    Western blot - ABflo® 647 Conjugated AIF1/IBA1 Rabbit mAb (A26033)

    Western blot analysis of various lysates using ABflo® 647 Conjugated AIF1/IBA1 Rabbit mAb (A26033) at 1:5000 dilution incubated overnight at 4℃.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.
    ABclonal:Immunofluorescence - ABflo® 647 Conjugated AIF1/IBA1 Rabbit mAb (A26033)}

    Immunofluorescence - ABflo® 647 Conjugated AIF1/IBA1 Rabbit mAb (A26033)

    Confocal imaging of paraffin-embedded Human brain tissue using ABflo® 647 Conjugated AIF1/IBA1 Rabbit mAb (A26033, dilution 1:500). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.
    ABclonal:Immunofluorescence - ABflo® 647 Conjugated AIF1/IBA1 Rabbit mAb (A26033)}

    Immunofluorescence - ABflo® 647 Conjugated AIF1/IBA1 Rabbit mAb (A26033)

    Confocal imaging of paraffin-embedded Rat brain tissue using ABflo® 647 Conjugated AIF1/IBA1 Rabbit mAb (A26033, dilution 1:500). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.
    ABclonal:Immunofluorescence - ABflo® 647 Conjugated AIF1/IBA1 Rabbit mAb (A26033)}

    Immunofluorescence - ABflo® 647 Conjugated AIF1/IBA1 Rabbit mAb (A26033)

    Confocal imaging of paraffin-embedded Mouse brain tissue using ABflo® 647 Conjugated AIF1/IBA1 Rabbit mAb (A26033, dilution 1:500). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.

    * For research use only. Not for therapeutic or diagnostic purposes.

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