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ABflo® 647 Rabbit anti-Human CD300c mAb (A24098)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Immunofluorescence - ABflo® 647 Rabbit anti-Human CD300c mAb (A24098)

Confocal imaging of 293T cells transfected with CD300c using ABflo® 647 Rabbit anti-Human CD300c mAb (A24098, dilution 1:100). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD300c mAb (A24098)

Flow cytometry: 1X10^6 293F cells (negative control, left) and 293F (Transfection, right) cells were surface-stained with ABflo® 647 Rabbit anti-Human CD300c mAb (A24098, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD300c mAb (A24098)

Flow cytometry: 1X10^6 293F (Transfection) cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human CD300c mAb (A24098, 5 μl/Test, right).

Overview

Product nameABflo® 647 Rabbit anti-Human CD300c mAb
Catalog No.A24098
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC64148-ABflo647
The CMRF35 antigen, which was identified by reactivity with a monoclonal antibody, is present on monocytes, neutrophils, and some T and B lymphocytes (Jackson et al., 1992 [PubMed 1349532]).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 21-183 of human CD300C (NP_006669.1).
SequenceGYFPLSHPMTVAGPVGGSLSVQCRYEKEHRTLNKFWCRPPQILRCDKIVETKGSAGKRNGRVSIRDSPANLSFTVTLENLTEEDAGTYWCGVDTPWLRDFHDPIVEVEVSVFPAGTTTASSPQSSMGTSGPPTKLPVHTWPSVTRKDSPEPSPHPGSLFSNVR
Gene ID
Swiss Prot
SynonymsLIR; CLM-6; CMRF35; IGSF16; CMRF-35; CMRF35A; CMRF-35A; CMRF35A1; CMRF35-A1
Calculated MW25kDa
Observed MW
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IF/ICC 1:50 - 1:200
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationImmunofluorescence    Flow Cytometry    
Positive samples
Cellular locationCell membrane, Single-pass type I membrane protein.

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ABclonal:Immunofluorescence - ABflo® 647 Rabbit anti-Human CD300c mAb (A24098)}

Immunofluorescence - ABflo® 647 Rabbit anti-Human CD300c mAb (A24098)

Confocal imaging of 293T cells transfected with CD300c using ABflo® 647 Rabbit anti-Human CD300c mAb (A24098, dilution 1:100). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD300c mAb (A24098)}

Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD300c mAb (A24098)

Flow cytometry: 1X10^6 293F cells (negative control, left) and 293F (Transfection, right) cells were surface-stained with ABflo® 647 Rabbit anti-Human CD300c mAb (A24098, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD300c mAb (A24098)}

Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD300c mAb (A24098)

Flow cytometry: 1X10^6 293F (Transfection) cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human CD300c mAb (A24098, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)